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Merck
CN

38371

Atto 488 Protein Labeling Kit

BioReagent, suitable for fluorescence

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
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product line

BioReagent

Quality Level

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 488 nm; λem 520 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

suitability

suitable for fluorescence

storage temp.

2-8°C

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General description

This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 488 Protein Labeling Kit is used to produce Atto 488 labeled proteins via conjugation to primary amine groups.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sheng Shu et al.
Nature communications, 13(1), 4576-4576 (2022-08-06)
Lipopolysaccharide (LPS) is an essential glycolipid and forms a protective permeability barrier for most Gram-negative bacteria. In E. coli, LPS levels are under feedback control, achieved by FtsH-mediated degradation of LpxC, which catalyzes the first committed step in LPS synthesis.
Andrea Pallares Pallares et al.
Food & function, 9(12), 6544-6554 (2018-11-28)
The presence of cell walls entrapping starch granules in common bean cotyledons, prevailing after thermal processing and mechanical disintegration, has been identified as the main reason for their (s)low in vitro starch digestibility. Nevertheless, it is unknown if the role
Bruno da Rocha-Azevedo et al.
Cell reports, 32(13), 108187-108187 (2020-10-01)
The dynamic nanoscale organization of cell surface receptors plays an important role in signaling. We determine this organization and its relation to activation of VEGF receptor-2 (VEGFR-2), a critical receptor tyrosine kinase in endothelial cells (ECs), by combining single-molecule imaging
Staffan Holmqvist et al.
Stem cell research, 15(1), 203-220 (2015-06-24)
Astrocytes play a critical role during the development and the maintenance of the CNS in health and disease. Yet, their lack of accessibility from fetuses and from the brain of diseased patients has hindered our understanding of their full implication
Lei Cao et al.
Science advances, 7(5) (2021-02-12)
Na+/K+-ATPase (NKA) plays important roles in maintaining cellular homeostasis. Conversely, reduced NKA activity has been reported in aging and neurodegenerative diseases. However, little is known about the function of NKA in the pathogenesis of Parkinson's disease (PD). Here, we report

Articles

Protein labeling kits with Atto and Tracy dyes provide easy fluorescent labeling of purified proteins, enzymes, and antibodies.

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