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Merck
CN

38371

Atto 488 Protein Labeling Kit

BioReagent, suitable for fluorescence

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Storage temp.:
2-8°C
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product line

BioReagent

Quality Level

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 488 nm; λem 520 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

suitability

suitable for fluorescence

storage temp.

2-8°C

General description

This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 488 Protein Labeling Kit is used to produce Atto 488 labeled proteins via conjugation to primary amine groups.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.


Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Articles

Protein labeling kits with Atto and Tracy dyes provide easy fluorescent labeling of purified proteins, enzymes, and antibodies.


Sheng Shu et al.
Nature communications, 13(1), 4576-4576 (2022-08-06)
Lipopolysaccharide (LPS) is an essential glycolipid and forms a protective permeability barrier for most Gram-negative bacteria. In E. coli, LPS levels are under feedback control, achieved by FtsH-mediated degradation of LpxC, which catalyzes the first committed step in LPS synthesis.
Andrea Pallares Pallares et al.
Food & function, 9(12), 6544-6554 (2018-11-28)
The presence of cell walls entrapping starch granules in common bean cotyledons, prevailing after thermal processing and mechanical disintegration, has been identified as the main reason for their (s)low in vitro starch digestibility. Nevertheless, it is unknown if the role
Gražvydas Lukinavičius et al.
Current opinion in chemical biology, 15(6), 768-774 (2011-11-15)
Numerous synthetic fluorophores have been developed that can switch their spectroscopic properties upon interaction with other molecules or by irradiation with light. In recent years, protein-labeling techniques have been introduced that permit the specific attachment of such molecules to proteins



Global Trade Item Number

SKUGTIN
38371-1KT04061826722749