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388R-1

Nestin (EP287) Rabbit Monoclonal Primary Antibody

Name: Nestin (EP287) Rabbit Monoclonal Primary Antibody
Brand: SIGMA

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About This Item

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

EP287, monoclonal

Application:

IHC (p)

Citations:

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biological source

rabbit

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

EP287, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (388R-14), vial of 0.5 mL concentrate (388R-15), vial of 1.0 mL concentrate (388R-16), vial of 1.0 mL predilute ready-to-use (388R-17), vial of 7.0 mL predilute ready-to-use (388R-18)

manufacturer/tradename

Cell Marque^®

concentration

0.1-0.5 μg/mL (predilute), 50-500 μg/mL (concentrate)

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500 (concentrated)

isotype

IgG

control

tonsil

shipped in

wet ice

storage temp.

2-8°C

visualization

cytoplasmic

Gene Information

human ... NES(10763)

General description

Nestin is a class VI intermediate filament (IF) protein. Nestin is expressed in neural progenitor cells during development of the central nervous system and peripheral nervous system. Cytoplasmic anti-nestin staining mostly occurs under pathological conditions. It has been reported that nestin expression is significantly increased in melanoma and correlated with more advanced stages of the disease. Nestin immunoreactivity is also reported in melanoma cells of HMB-45-negative amelanotic and melanotic, non-desmoplastic melanoma. Nestin is also useful in subclassifying breast carcinoma. Only cytoplasmic staining is considered positive, whereas any nuclear staining is considered as background artifact.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

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Analysis Note

Canada - IVD
European Union - IVD
Japan - RUO
United States - IVD

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

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Nestin is expressed in the basal/myoepithelial layer of the mammary gland and is a selective marker of basal epithelial breast tumors.

Hua Li et al.

Cancer research, 67(2), 501-510 (2007-01-20)

Transcriptional profiling has identified five breast cancer subtypes, of which the basal epithelial is most aggressive and correlates with poor prognosis. These tumors display a high degree of cellular heterogeneity and lack established molecular targets, such as estrogen receptor-alpha, progesterone

Nestin expression in cutaneous melanomas and melanocytic nevi.

Svetlana Brychtova et al.

Journal of cutaneous pathology, 34(5), 370-375 (2007-04-24)

Nestin is one of the intermediate filaments that are expressed in proliferating neural progenitor cells during development of the central nervous system (CNS) and peripheral nervous system. Postnatal re-expression of the protein occurs mainly under pathological conditions, including injury and

Nestin is expressed in HMB-45 negative melanoma cells in dermal parts of nodular melanoma.

Maho Kanoh et al.

The Journal of dermatology, 37(6), 505-511 (2010-06-12)

Nestin, a marker of neural stem cells, is expressed in the stem cells of the mouse hair follicle. The nestin-expressing hair follicle stem cells can differentiate into neurons, glia, keratocytes, smooth muscle cells and melanocytes in vitro. These pluripotent nestin-expressing

Global Trade Item Number

SKU	GTIN
388R-14-RUO	04061837570957
388R-15-RUO	04061837570971
388R-17	04061837571008
388R-15	04061837570964
388R-17-RUO	04061837571015
388R-14	04061837570940
388R-16	04061837570988
388R-16-RUO	04061837570995
388R-18	04061837571022
388R-18-RUO	04061837571039

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