38922
Abberior® STAR 512, NHS ester
for STED application
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Quality Level
assay
≥80.0% (degree of coupling)
mol wt
Mw 836.7 g/mol
solubility
DMF: 0.25 mg/mL, clear
fluorescence
λex 510 nm; λem 530 nm±5 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Absorption Maximum, λmax: 517 nm (MeOH),
511 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 85,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.24 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.07 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 533 nm (MeOH),
530 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 −620 nm
Fluorescence Quantum Yield, η: 0.82 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 4.1 ns (PBS, pH 7.4)
511 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 85,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.24 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.07 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 533 nm (MeOH),
530 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 −620 nm
Fluorescence Quantum Yield, η: 0.82 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 4.1 ns (PBS, pH 7.4)
Application
Abberior® Star 512 labelled phosphoethanolamine lipid analogues were used for gated STED-FCS (stimulated emission depletion - fluorescence correlation spectroscopy) study.
Designed and tested for fluorescent super-resolution microscopy
Other Notes
Legal Information
abberior is a registered trademark of Abberior GmbH
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Mathias P Clausen et al.
Methods (San Diego, Calif.), 88, 67-75 (2015-07-01)
Recent years have seen the development of multiple technologies to investigate, with great spatial and temporal resolution, the dynamics of lipids in cellular and model membranes. One of these approaches is the combination of far-field super-resolution stimulated-emission-depletion (STED) microscopy with
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Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
T A Klar et al.
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We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited