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Merck
CN

39304

Anti-Mouse IgG - Mega 520

Synonym(s):

Mega 520-Anti-Mouse-IgG

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About This Item

UNSPSC Code:
12352203
MDL number:
Technical Service
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biological source

goat

conjugate

Mega 520 conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

contains

50% glycerol as stabilizer

species reactivity

mouse

concentration

1 mg/mL protein

fluorescence

λex 527 nm; λem 658 nm in PBS

storage temp.

−20°C

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Affinity isolated antigen specific antibody is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Hence, Mega 520-goat anti-mouse IgG binds specifically to mouse IgGs.

Immunogen

purified mouse IgG

Application

Mega 520-goat anti-mouse IgG can be used for fluorescence-based immunoassays.

Physical form

Mega 520-goat anti-mouse IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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ppe

Eyeshields, Gloves

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Complement-fixing IgG1 constitutes a new subclass of mouse IgG.
P L Ey et al.
Nature, 281(5731), 492-493 (1979-10-11)
Structure and function of human and murine receptors for IgG.
J C Unkeless et al.
Annual review of immunology, 6, 251-281 (1988-01-01)

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