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Merck
CN

41969

5-Carboxy-X-rhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

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About This Item

Empirical Formula (Hill Notation):
C37H33N3O7
CAS Number:
209734-74-7
Molecular Weight:
631.67
NACRES:
NA.32
PubChem Substance ID:
329756168
UNSPSC Code:
12352116
MDL number:
MFCD07437880

Key Documents

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Product Name

5-Carboxy-X-rhodamine N-succinimidyl ester, BioReagent, suitable for fluorescence

InChI

1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(26(19-22)36(43)44)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2

SMILES string

[O-]C(=O)c1cc(ccc1-c2c3cc4CCCN5CCCc(c45)c3[o+]c6c7CCCN8CCCc(cc26)c78)C(=O)ON9C(=O)CCC9=O

InChI key

BTTBJYLMDASSAS-UHFFFAOYSA-N

product line

BioReagent

form

powder

solubility

DMF: soluble
acetonitrile: soluble

fluorescence

λex 575 nm; λem 600 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

100

Application

5-Carboxy-X-rhodamine N-succinimidyl ester (ROX-SE) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications.Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing.

Other Notes

Labeling reagent for prepn. of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM7490
BCCH0855
BCCC8679
BCCC2037
BCBZ9312

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Jonathan F Lovell et al.
Journal of the American Chemical Society, 133(46), 18580-18582 (2011-10-27)
Photodynamic therapy (PDT) stands to benefit from improved approaches to real-time treatment monitoring. One method is to use activatable photosensitizers that can both induce cell death (via singlet oxygen) and monitor it (via caspase detection). Here, we report porphyrins as
Yunfeng Xia et al.
The Analyst, 137(24), 5705-5709 (2012-10-27)
We report a sensitive approach for SERS detection of cytochrome c using target binding-induced conformational changes of signal transduction probe (STP). STP labeled with a SERS-active molecule, carboxy-X-rhodamine (ROX), is immobilized on the substrate where the formation of a rigid
Patrick J Finn et al.
Nucleic acids research, 30(13), 2877-2885 (2002-06-28)
A novel series of charge-modified, dye-labeled 2',3'-dideoxynucleoside-triphosphate terminators were synthesized and evaluated as reagents for DNA sequencing. These terminators possess an advantage over existing reagents in that no purification is required to remove unreacted nucleotide or associated breakdown products prior
Ting Qiu et al.
The Analyst, 135(9), 2394-2399 (2010-08-03)
A new strategy for quantitatively detecting micrococcal nuclease (MNase) is proposed using electrostatic interaction-based fluorescence resonance energy transfer (FRET) between positively charged QDs and negatively charged dye-labeled single-stranded DNA (dye-ssDNA). Herein, we have made our attempt to develop a strategy
Xia Xiang et al.
Analytica chimica acta, 751, 155-160 (2012-10-23)
The development of simple and inexpensive DNA detection strategy is very significant for droplet-based microfluidic system. Here, a droplet-based biosensor for multiplexed DNA analysis is developed with a common imaging device by using fluorescence-based colorimetric method and a graphene nanoprobe.
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