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Merck
CN

46394

Fluo 3-AM

suitable for fluorescence

Synonym(s):

4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4′-methyl-2,2′(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester

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About This Item

Empirical Formula (Hill Notation):
C51H50Cl2N2O23
CAS Number:
121714-22-5
Molecular Weight:
1129.85
UNSPSC Code:
12352200
PubChem Substance ID:
24870122

Key Documents

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SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc(ccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(OCOC(C)=O)c(Cl)cc35

solubility

DMSO: soluble

fluorescence

λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+

suitability

suitable for fluorescence

storage temp.

−20°C

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Application

Cell permeable fluorescent indicator of intracellular Ca2+; non-fluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+; lower binding affinity allows measurement of higher peaks of Ca2+ transients than with Fura 2.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBF8767V
437121/1
1227905
1126008
1100086

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