47614
Fluram
BioReagent, suitable for fluorescence, ≥99.0% (UV)
Synonym(s):
Fluorescamine, 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione
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About This Item
Empirical Formula (Hill Notation):
C17H10O4
CAS Number:
Molecular Weight:
278.26
EC Number:
253-814-5
UNSPSC Code:
12352200
PubChem Substance ID:
Beilstein/REAXYS Number:
921143
MDL number:
product line
BioReagent
assay
≥99.0% (UV)
mp
153-157 °C
solubility
acetonitrile: soluble, ethanol: soluble
fluorescence
λex 234 nm, λex 390 nm; λem 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)
suitability
suitable for fluorescence
SMILES string
O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
InChI
1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
InChI key
ZFKJVJIDPQDDFY-UHFFFAOYSA-N
Application
Fluram (fluorescamine) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides and other molecules to form stable, highly fluorescent compounds. Fluorescamine is useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes and as a pre-column derivatization reagent. It effectively blocks newly generated amino termini in protein sequence analyses.
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Legal Information
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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José Pedro Castro et al.
Redox biology, 21, 101108-101108 (2019-01-21)
Aging is accompanied by the accumulation of oxidized proteins. To remove them, cells employ the proteasomal and autophagy-lysosomal systems; however, if the clearance rate is inferior to its formation, protein aggregates form as a hallmark of proteostasis loss. In cells
Amit K Jha et al.
Biomaterials, 32(10), 2466-2478 (2011-01-11)
We have created hyaluronic acid (HA)-based, cell-adhesive hydrogels that direct the initial attachment and the subsequent differentiation of human mesenchymal stem cells (MSCs) into pre-osteoblasts without osteogenic supplements. HA-based hydrogel particles (HGPs) with an average diameter of 5-6 μm containing
Min Jia et al.
Analytical chemistry, 81(19), 8033-8040 (2009-10-01)
Fluorescamine derivatized 3-amino-2,2,5,5,-tetramethyl-1-pyrrolidinyloxy (I) is shown to undergo an irreversible reaction with peroxyl radicals and other radical oxidants to generate a more highly fluorescent diamagnetic product (II) and thus can be used as a highly sensitive and versatile probe to
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 47614-100MG-F | 04061833277331 |
| 47614-25MG-F | 04061832532219 |
| 47614-1G-F | 04061835570591 |