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Merck
CN

47614

Fluram

BioReagent, suitable for fluorescence, ≥99.0% (UV)

Synonym(s):

Fluorescamine, 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione

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About This Item

Empirical Formula (Hill Notation):
C17H10O4
CAS Number:
38183-12-9
Molecular Weight:
278.26
EC Number:
253-814-5
UNSPSC Code:
12352200
PubChem Substance ID:
329757329
Beilstein/REAXYS Number:
921143
MDL number:
MFCD00005928

Key Documents

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InChI key

ZFKJVJIDPQDDFY-UHFFFAOYSA-N

InChI

1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H

SMILES string

O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14

product line

BioReagent

assay

≥99.0% (UV)

solubility

acetonitrile: soluble, ethanol: soluble

fluorescence

λex 234 nm, λex 390 nm; λem 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)

suitability

suitable for fluorescence

Application

Fluram (fluorescamine) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides and other molecules to form stable, highly fluorescent compounds. Fluorescamine is useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes and as a pre-column derivatization reagent. It effectively blocks newly generated amino termini in protein sequence analyses.
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB8644
BCBZ9346
BCBW5138
047M4054V
BCBS5268V

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Khin Yin Win et al.
The Analyst, 137(10), 2328-2332 (2012-03-13)
Here we report a fast and effective method to visualize interactive proteins across intact mammalian cells via on-site formation of fluorescence using instant reaction of non-fluorescent fluorescamine with primary amines on proteins. Without interference by fluorescence background, this fluorogenic labelling
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