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Merck
CN

47860

Basic Fuchsin

Synonym(s):

Basic Violet 14, Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-2-methyl hydrochloride, Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-2-methyl

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About This Item

Empirical Formula (Hill Notation):
C20H20ClN3
CAS Number:
632-99-5
Molecular Weight:
337.85
NACRES:
NA.47
PubChem Substance ID:
24871527
UNSPSC Code:
12171500
Colour Index Number:
42510

Key Documents

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InChI

1S/C20H19N3.ClH/c1-13-12-16(6-11-19(13)23)20(14-2-7-17(21)8-3-14)15-4-9-18(22)10-5-15;/h2-12,23H,21-22H2,1H3;1H

SMILES string

NC1=CC=C(C(C2=CC(C)=C(N)C=C2)=C3C=CC(C=C3)=N)C=C1.[Cl]

InChI key

AXDJCCTWPBKUKL-UHFFFAOYSA-N

suitability

suitable for microscopy

application(s)

environmental

General description

Basic fuchsin, a triphenylmethane dye is commonly a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II (no CI number) and new fuchsine (CI 42520). Basic fuchsin is widely used in tissue-specific staining of biological and coloring several materials including orlon, cotton, leather, paper etc. It is also used to stain collagen, muscle and mitochondria.

Application

Basic Fuchsin has been used in several biological tests including:
  • Acid-fast staining with carbol-fuchsine
  • Periodic Acid-Schiff method
  • Feulgen technique for DNA staining
  • ENDO culture medium for distinguishing between the bacteria Escherichia coli and Aerobacter aerogenes
  • Leifson′s method for bacterial flagella staining.

Biochem/physiol Actions

Basic fuchsin possesses anesthetic, bactericidal (gram-positive), and fungicidal properties.

Analysis Note

homologous mixture of dyes, contains dominantly (~35-40%) Basic Fuchsin (C.I. 42510)

pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H351

Hazard Classifications

Carc. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB8821
BCCB5714
BCCB4084
BCCB4085
BCBW9753

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Daria Bloch et al.
Development (Cambridge, England), 146(17) (2019-08-11)
Patterning of the root xylem into protoxylem (PX) and metaxylem is regulated by auxin-cytokinin signaling and microRNA miR165a/166b-mediated suppression of genes encoding Class III HOMEODOMAIN LEU-ZIPPER (HD-ZIPIII) proteins. We found that, in Arabidopsis, osmotic stress via core abscisic acid (ABA)

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