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Merck
CN

49328

Sigma-Aldrich

EVOblue™ 30

≥90.0% (coupling to amines)

Synonym(s):

3-[N-(2-Carboxyethyl)methylamino]-7-[N-ethyl(3-sulfonatopropyl)amino]phenoxazin-5-ium

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About This Item

Empirical Formula (Hill Notation):
C21H25N3O6S
CAS Number:
343257-52-3
Molecular Weight:
447.50
MDL number:
MFCD03791081
UNSPSC Code:
12352200
PubChem Substance ID:
329757411

Key Documents

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Assay

≥90.0% (coupling to amines)

solubility

DMF: soluble

fluorescence

λex 635 nm; λem 666 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCN(CCCS([O-])(=O)=O)c1ccc2nc3ccc(cc3[o+]c2c1)N(C)CCC(O)=O

InChI

1S/C21H25N3O6S/c1-3-24(10-4-12-31(27,28)29)16-6-8-18-20(14-16)30-19-13-15(5-7-17(19)22-18)23(2)11-9-21(25)26/h5-8,13-14H,3-4,9-12H2,1-2H3,(H-,25,26,27,28,29)

InChI key

RCTKCVWZEGPPCK-UHFFFAOYSA-N

Application

EVOblue™ 30 is a fluorescent dye for covalently labeling a wide range of biological molecules. The lower excitation energy needed for EVOblue™ 30 causes less photodestruction and exceeds the lifetime of the fluorescent signal compared to common dyes. EVOblue™ 30 is optimized for an excitation at 635 nm and an emission around 670 nm - both allowing the use of common laser and filter sets. In contrast to commercially available red excitable dyes, EVOblue™ 30 reveals a high chemical stability and suitability for high performance fluorescence applications.

Features and Benefits

EVOblue® 30 is a fluorescent dye for covalently labeling a wide range of biological molecules. The lower excitation energy needed for EVOblue 30 causes less photodestruction and exceeds the lifetime of the fluorescent signal compared to common dyes. EVOblue 30 is optimized for an excitation at 635 nm and an emission around 670 nm - both allowing the use of common laser and filter sets. In contrast to commercially available red excitable dyes, EVOblue 30 reveals a high chemical stability and suitablity for high performance fluorescence applications.

Other Notes

Fluorescent marker for the red spectral region which is very stable

Legal Information

EVOblue is a trademark of Evotec Technologies GmbH

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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C.E. Lopez, J. Fries
Bioforum, 24, 32-32 (2001)

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