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Merck
CN

50277

5,6-Diaminofluorescein diacetate

BioReagent, suitable for fluorescence, 98% (HPCE)

Synonym(s):

4,5-Diaminofluorescein diacetate, DAF-2 DA

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About This Item

Empirical Formula (Hill Notation):
C24H18N2O7
CAS Number:
205391-02-2
Molecular Weight:
446.41
NACRES:
NA.32
PubChem Substance ID:
57651247
UNSPSC Code:
12352200
MDL number:
MFCD05865101

Key Documents

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InChI

1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(N)c(N)cc45)c1

InChI key

PTSUYDXEEKDBQU-UHFFFAOYSA-N

product line

BioReagent

assay

98% (HPCE)

fluorescence

λex 491 nm; λem 513 nm

suitability

suitable for fluorescence

Application

5,6-Diaminofluorescein diacetate (DAF-2 DA), a cell permeable analog of DAF-2, is used in intracellular fluorimetric detection of nitric oxide/NOS production.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBZ9982
BCBZ3652
BCBR0791V
BCBQ9166V

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H Kojima et al.
Neuroreport, 9(15), 3345-3348 (1998-12-17)
The biological functions of nitric oxide in the neuronal system remain controversial. Using a novel fluorescence indicator, DAF-2 DA, for direct detection of NO, we examined both acute rat brain slices and organotypic culture of brain slices to ascertain NO
Andréa C Celotto et al.
European journal of pharmacology, 656(1-3), 88-93 (2011-02-09)
We investigated the mechanism by which extracellular acidification promotes relaxation in rat thoracic aorta. The relaxation response to HCl-induced extracellular acidification (7.4 to 6.5) was measured in aortic rings pre-contracted with phenylephrine (Phe, 10(-6) M) or KCl (45mM). The vascular
H Kojima et al.
Analytical chemistry, 70(13), 2446-2453 (1998-07-17)
Nitric oxide is a gaseous, free radical which plays a role as an intracellular second messenger and a diffusable intercellular messenger. To obtain direct evidence for NO functions in vivo, we have designed and synthesized diaminofluoresceins (DAFs) as novel fluorescent
Shoji Tsuji et al.
Journal of pediatric hematology/oncology, 34(7), 500-502 (2012-09-01)
Chronic granulomatous disease (CGD) is an inherited disorder in which phagocytic leukocytes fail to generate superoxide (O(2)(-)) and antimicrobial oxidants. Patients with CGD develop recurrent and often life-threatening infections due to catalase-positive microorganisms. We attempted to measure the production of
Juan L Turrion-Gomez et al.
Molecular plant pathology, 12(6), 606-616 (2011-07-05)
Nitric oxide (NO) production by Botrytis cinerea and the effect of externally supplied NO were studied during saprophytic growth and plant infection. Fluorescence analysis with 4,5-diaminofluorescein diacetate and electrochemical studies were conducted in vitro between 4 and 20 h of
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