5107
ICAM2 human
recombinant, expressed in E. coli, 0.5 mg protein/mL
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥90% (SDS-PAGE)
Form:
liquid
biological source
human
recombinant
expressed in E. coli
description
0.1 mg recombinant human ICAM2 in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
sterility
Filtered sterilized solution
assay
≥90% (SDS-PAGE)
form
liquid
packaging
pkg of 100 μg
concentration
0.5 mg protein/mL
accession no.
NP_000864
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... ICAM2(3384)
Application
Coating a plate well (6 well plate) with this recombinant ICAM2 protein in neuronal cell specific medium at 1-10 μg / well allows for 1) human lymphocyte cell / receptor interaction studies in vitro and 2) use as a culture matrix protein for anti-tumor immuno-response studies in vitro.
Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
1. Thaw ICAM2 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly.
Note: Use 1 ml PBS per well in a 6-well plate.
2. Add 1 - 10 μg protein to each well and incubate at 2-10 °C overnight.
3. After incubation, aspirate remaining material.
4. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained.
Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
1. Thaw ICAM2 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly.
Note: Use 1 ml PBS per well in a 6-well plate.
2. Add 1 - 10 μg protein to each well and incubate at 2-10 °C overnight.
3. After incubation, aspirate remaining material.
4. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained.
Preparation Note
The full-length extracellular domain of human ICAM2 gene (25-223 aa) was constructed with 29 N-terminal T7/HIS-tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.
Other Notes
MASMTGGQQMGRGHHHHHHGNLYFQGGEFELKVFEVHVRPKKLAVEPKGSLEVNCSTTCNQPEVGGLETSLDKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQ
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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