Atto Rho11 maleimide is a new rhodamine dye featuring a functionality for coupling to bio-molecules such as DNA, RNA, proteins etc. The label shows very high fluorescence efficiency and exceptionally high photostability. The dye is moderately hydrophilic. ATTO Rho11 is a cationic dye. After coupling to a substrate the label carries a net electrical charge of +1. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides react more selectively with thiols. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.