52944
Anti-Rabbit IgG−Abberior® STAR 488 antibody produced in goat
for STED application
Synonym(s):
Abberior® STAR 488-Anti-Rabbit IgG antibody produced in goat
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Product Name
Anti-Rabbit IgG−Abberior® STAR 488 antibody produced in goat, for STED application
biological source
goat
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rabbit
availability
available only in USA and Canada
concentration
~1 mg/mL
fluorescence
λex 503 nm; λem 524 nm in PBS, pH 7.4
storage temp.
−20°C
Quality Level
Analysis Note
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Application
Anti-Rabbit IgG-Abberior® STAR 488 antibody has been used as a secondary antibody:
- for fluorescent immunohistochemistry in prostate cancer cell lines, PPC-1 and TSU-Pr1
- for immunofluorescence in HeLa and monkey kidney COS-7 cells
- for STED (stimulated emission depletion) microscopy in OLN-t40-α-syn cells (oligodendroglial cells)
Designed and tested for fluorescent super-resolution microscopy
General description
Abberior STAR 488 was developed for STED and confocal microscopy in the green spectral region. It is a bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with the prominent 488 nm laser line. For STED microscopy, Abberior STAR 488 can be efficiently used with STED Laser wavelengths between 590 - 610 nm. Abberior STAR 488 can substitute dyes like Oregon Green 488, Atto™ 488 or Alexa Fluor™ 488. Abberior STAR 488 is the ideal partner for Abberior STAR 440SXP to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 503 (PBS pH 7.4), 504 (H2O), 507 (MeOH + 0.1% TFA)
Extinction Coefficient, εmax [M-1cm-1]: 65 000 (PBS pH 7.4), 80 000 (H2O), 85 000 (MeOH + 0.1% TFA)
Correction Factor, CF260 = ε260/εmax: 0,28 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,14 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 524 (PBS pH 7.4), 525 (H2O), 531 (MeOH + 0.1% TFA)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,89 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,9 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 503 (PBS pH 7.4), 504 (H2O), 507 (MeOH + 0.1% TFA)
Extinction Coefficient, εmax [M-1cm-1]: 65 000 (PBS pH 7.4), 80 000 (H2O), 85 000 (MeOH + 0.1% TFA)
Correction Factor, CF260 = ε260/εmax: 0,28 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,14 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 524 (PBS pH 7.4), 525 (H2O), 531 (MeOH + 0.1% TFA)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,89 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,9 (PBS pH 7.4)
Other Notes
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Combination of axitinib and dasatinib for anti-cancer activities in two prostate cancer cell lines.
Peng N
Bangladesh Journal of Pharmacology, 11, 10-10 (2016)
Giuseppe Vicidomini et al.
Methods (San Diego, Calif.), 66(2), 124-130 (2013-07-03)
Stimulation emission depletion (STED) microscopy breaks the spatial resolution limit of conventional light microscopy while retaining its major advantages, such as working under physiological conditions. These properties make STED microscopy a perfect tool for investigating dynamic sub-cellular processes in living
Katharina Pukaß et al.
Frontiers in cellular neuroscience, 9, 163-163 (2015-05-23)
α-Synuclein (α-syn) positive glial cytoplasmic inclusions (GCI) originating in oligodendrocytes (ODC) are a characteristic hallmark in multiple system atrophy (MSA). Their occurrence may be linked to a failure of the ubiquitin proteasome system (UPS) or the autophagic pathway. For proteasomal
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
S W Hell et al.
Optics letters, 19(11), 780-782 (1994-06-01)
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation
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52944, Anti-Rabbit IgG-Abberior STAR 488
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