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54459

HEPES

Name: HEPES
Brand: SIGMA

BioUltra, ≥99.5% (T)

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):

C₈H₁₈N₂O₄S

CAS Number:

7365-45-9

Molecular Weight:

238.30

EC Number:

230-907-9

UNSPSC Code:

12161700

PubChem Substance ID:

24878775

Beilstein/REAXYS Number:

883043

MDL number:

MFCD00006158

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Properties

product line

BioUltra

assay

≥99.5% (T)

impurities

insoluble matter, passes filter test

ign. residue

≤0.1% (as SO₄)

loss

≤0.5% loss on drying, 110 °C

pH

5.0-6.5 (25 °C, 1 M in H₂O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H₂O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤50 mg/kg, sulfate (SO₄^2-): ≤200 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

1 M in H₂O

UV absorption

λ: 260 nm A_max: 0.045, λ: 280 nm A_max: 0.035

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Other Notes

One of the best general purpose buffers available for biological research. Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, CO₂ fixation; Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates; Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials.

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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