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Merck
CN

56659C

SAFC

Ham′s Nutrient Mixture F12

with 1.0 mM L-glutamine, without sodium bicarbonate, dry powder

Synonym(s):

Nutrient Mixture F12 Ham’s

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352200

Key Documents

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description

for research or for further manufacturing use

form

dry powder

quality

for further manufacturing use

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

components

phenol red: 1.274 mg/L
L-glutamine: 146.2 mg/L
sodium pyruvate: 110.1 mg/L

shipped in

ambient

storage temp.

2-8°C

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Application

Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.

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Lei Tong et al.
Biochemical and biophysical research communications, 401(4), 592-597 (2010-10-06)
Neural stem cells (NSCs) are multipotent stem cells that have the potential to differentiate into different cells of the neural lineage like neurons, astrocytes and oligodendrocytes. In the current work, we explored whether NSCs could be differentiated into functional Schwann-like
S Mahmoudi et al.
Cell death & disease, 2, e114-e114 (2011-03-04)
We previously identified WRAP53 as an antisense transcript that regulates the p53 tumor suppressor. The WRAP53 gene also encodes a protein essential for Cajal body formation and involved in cellular trafficking of the survival of motor neuron complex, the telomerase

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