56659C
Ham′s Nutrient Mixture F12
with 1.0 mM L-glutamine, without sodium bicarbonate, dry powder
Synonym(s):
Nutrient Mixture F12 Ham’s
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About This Item
UNSPSC Code:
12352200
MDL number:
description
for research or for further manufacturing use
form
dry powder
quality
for further manufacturing use
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
components
phenol red: 1.274 mg/L
L-glutamine: 146.2 mg/L
sodium pyruvate: 110.1 mg/L
shipped in
ambient
storage temp.
2-8°C
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Application
Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.
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