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Merck
CN

57672

N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid

suitable for fluorescence, ≥95% (HPLC)

Synonym(s):

1,5-I-AEDANS, 5-[2-(Iodoacetamido)ethylamino]naphthalene-1-sulfonic acid, N-Iodoacetyl-N′-(5-sulfo-1-naphthyl)ethylenediamine, Hudson-Weber-Reagent

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About This Item

Linear Formula:
ICH2CONHCH2CH2NHC10H6SO3H
CAS Number:
Molecular Weight:
434.25
UNSPSC Code:
12352200
PubChem Substance ID:
eCl@ss:
32035301
EC Number:
253-277-7
Beilstein/REAXYS Number:
3004454
MDL number:
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assay

≥95% (HPLC)

fluorescence

λex 336 nm; λem 490 nm (pH 8.0), λex 337 nm; λem 498 nm (after derivatization with 2-mercaptoethanol)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

OS(=O)(=O)c1cccc2c(NCCNC(=O)CI)cccc12

InChI

1S/C14H15IN2O4S/c15-9-14(18)17-8-7-16-12-5-1-4-11-10(12)3-2-6-13(11)22(19,20)21/h1-6,16H,7-9H2,(H,17,18)(H,19,20,21)

InChI key

ZMERMCRYYFRELX-UHFFFAOYSA-N

Application

N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid (1,5-I-AEDANS) is a fluorescence probe used to label protein sulfhydryls to study conformational changes, fluorescence anisotropy and protein:protein interactions.

Other Notes

Fluorescent sulfhydryl reagent


Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Samrat Mukhopadhyay et al.
Journal of molecular biology, 358(4), 935-942 (2006-03-21)
The small protein barstar aggregates at low pH to form soluble oligomers, which can be transformed into fibrillar aggregates at an elevated temperature. To characterize structurally, with residue-specific resolution, the process of amyloid formation of barstar, as well as to
Jason R Waggoner et al.
Biochemistry, 46(7), 1999-2009 (2007-01-31)
We have used steady-state fluorescence spectroscopy in combination with enzyme kinetic assays to test the hypothesis that phospholamban (PLB) stabilizes the Ca-ATPase in the E2 intermediate state. The cardiac muscle Ca-ATPase (SERCA2a) isoform was expressed either alone or coexpressed with
Torsten Heidenreich et al.
The Journal of biological chemistry, 280(6), 4213-4218 (2004-11-25)
The molybdenum cofactor sulfurase ABA3 from Arabidopsis thaliana specifically regulates the activity of the molybdenum enzymes aldehyde oxidase and xanthine dehydrogenase by converting their molybdenum cofactor from the desulfo-form into the sulfo-form. ABA3 is a two-domain protein with an NH2-terminal