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Merck
CN

61305

Penicillinase from Bacillus cereus

lyophilized, powder, white, ~13 U/mg

Synonym(s):

β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase

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About This Item

CAS Number:
9073-60-3
EC Number:
3.5.2.6 (BRENDA, IUBMB)
MDL number:
MFCD00131819
UNSPSC Code:
12352204
NACRES:
NA.54

Key Documents

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biological source

Bacillus sp. (Bacillus cereus)

Quality Level

100

form

powder

quality

lyophilized

specific activity

~13 U/mg

mol wt

Mr ~30000

technique(s)

activity assay: suitable

color

white

suitability

suitable for component for culture media

application(s)

diagnostic assay manufacturing

foreign activity

β-lactamase II ≤2%

storage temp.

−20°C

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General description

Penicillinase from Bacillus cereus is both an extracellular and cell-bound enzyme. It has a single polypeptide chain and has a molecular weight of 31 kDa. It lacks cysteine in the protein sequence.

Application

Penicillinase from Bacillus cereus may be used in the preparation of biosensor for amperometric detection of hydrolysis of penicillin.
Used in the production of penicillin.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
Used to destroy β-lactam antibiotics in body fluids or culture media. For the determination of penicillin; In the coupled enzyme assay for isopenicillin N synthetase; ELISA of estradiol; Penicillinase optodes.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL1159
BCCM2918
BCCK6124
BCCJ0566
BCCD7047

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J E Baldwin et al.
Analytical biochemistry, 145(1), 183-187 (1985-02-15)
The development of a coupled enzyme assay for the determination of isopenicillin N synthetase activity in purified extracts from Cephalosporium acremonium was described. Isopenicillin N formed from its precursor, delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV), by the synthetase was hydrolyzed by beta-lactamase I to
W. Hobel et al.
Biosensors And Bioelectronics, 7, 549-549 (1992)
Flow injection determination of penicillins using immobilized penicillinase in a single bead string reactor.
R Gnanasekaran et al.
Analytical chemistry, 57(6), 1005-1009 (1985-05-01)
T. Scheper et al.
Analytica Chimica Acta, 163, 111-111 (1984)
P K Pandey et al.
Clinica chimica acta; international journal of clinical chemistry, 190(3), 175-184 (1990-10-15)
An enzyme-linked immunosorbant assay (ELISA) for measuring oestradiol directly in plasma without extraction utilizing antibodies raised against oestradiol-3-(O-carboxymethyl) ether-bovine serum albumin conjugate, and oestradiol-6-(O-carboxymethyl) oxime linked to penicillinase (EC 3.5.2.6) as a marker was developed. Polyvinyl 96-well microtitre plates were
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