61761
Lectin-Fluorescein isothiocyanate conjugate from Canavalia ensiformis
lyophilized, powder
Synonym(s):
Concanavalin A, FITC-labeled, FITC-Con A
form
powder
quality
lyophilized
mol wt
Mr ~53000
extent of labeling
≥3 mol/mol FITC protein (substitution)
fluorescence
λex 495 nm; λem 518 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
storage temp.
2-8°C
Application
Nerve cells accumulate certain FITC-labelled lectins, with much greater accumulation in nerves that are regnerating after traumatic damage. Uptake is seen with Con A and wheat germ agglutinin, but not agglutinins from Dolichos biflorus, peanut, Ulex europaeus, or soybean.
Physical form
lyophilized powder, containing phosphate buffer salts and NaCl
Other Notes
Staining of glycoproteins in polyacrylamide and agarose gels with fluorescent lectins
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Staining of glycoproteins in polyacrylamide and agarose gels with fluorescent lectins.
M Furlan et al.
Analytical biochemistry, 96(1), 208-214 (1979-07-01)
I Nennesmo
Journal of neurocytology, 12(6), 1007-1016 (1983-12-01)
Cytofluorometric quantification of the accumulation of somatopetally transported fluoroisothiocyanate (FITC)-labelled wheat germ agglutinin (WGA) and Concanavalin A (Con A) were performed on sections of the mouse facial nucleus. The lectins were injected bilaterally in the snout region of six-week-old mice
Gel protein stains: glycoproteins.
J E Gander
Methods in enzymology, 104, 447-451 (1984-01-01)
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