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Merck
CN

62300

Sigma-Aldrich

Lipase from porcine pancreas

powder, 15-35 U/mg

Synonym(s):

PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

CAS Number:
9001-62-1
EC Number:
3.1.1.3 (BRENDA, IUBMB)
EC Number:
232-619-9
MDL number:
MFCD00131509
UNSPSC Code:
12352204

Key Documents

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form

powder

specific activity

15-35 U/mg

mol wt

Mr ~50000

shipped in

wet ice

storage temp.

−20°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from triglycerides per minute at pH 8.0 and 37 °C (olive oil as substrate)
Selective acylation of primary alcohols in organic solvents; Enantioselective transesterification; Procedure for the removal of lipid contaminants in commercial lipases; Prepared without activation of other naturally occurring zymogens, partly soluble in water or buffer.; Use in the continuous synthesis of glycerides

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBH6515V
BCBC7768
1423710
1366256

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S. Misra et al.
Lipids, 19, 302-302 (1984)
J. Shield Wallace et al.
The Journal of Organic Chemistry, 55, 3544-3544 (1990)
M.M. Hoq et al.
Journal of the American Oil Chemists' Society, 61, 776-776 (1984)
S. Ramaswamy et al.
Tetrahedron Letters, 31, 3405-3405 (1990)

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