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62309

Sigma-Aldrich

Lipase from Pseudomonas cepacia

powder, light beige, ≥30 U/mg

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Synonym(s):
PCL, PS Lipase, Triacylglycerol acylhydrolase, Triacylglycerol lipase
CAS Number:
9001-62-1
Enzyme Commission number:
3.1.1.3 (BRENDA, IUBMB)
EC Number:
232-619-9
MDL number:
MFCD00131509
NACRES:
NA.54

biological source

bacterial (Pseudomonas cepacia)

Quality Level

100

form

powder

specific activity

≥30 U/mg

storage condition

(Tightly closed. Dry)

technique(s)

cell based assay: suitable

color

light beige

solubility

H2O: 2 mg/mL, hazy, faintly yellow

UniProt accession no.

A0A0J5WKE3

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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General description

Research area: cell-signaling

Lipase is a hydrolytic enzyme, found ubiquitously in nature. It belongs to the α/β-hydrolases fold family. Lipase structure contains amphipathic helical lid domain in the active site that helps in interfacial activation of protein.

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipase from Pseudomonas cepacian has been used to:
  • catalyze the degradation of polycaprolactone scaffold
  • catalyze the hydrolysis of Morita-Baylis-Hillman acetates during enzymatic kinetic resolution of racemic Morita-Baylis-Hillman adducts
  • as a standard for the generation of a calibration curve to determine the activity of lipase produced by microorganisms isolated from sludge derived from an urban wastewater treatment plant for ethanol production.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of carboxylic ester bonds in triacylglycerols to yield glycerol and free fatty acids. Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). Triacylglycerol lipases specifically hydrolyze the outer links of triacylglycerols and operate exclusively on the water-lipid interface. Lipolytic products and intermediates formed during lipolysis are involved in various cell-signaling processes. Lipases have broad substrate specificity and high enantioselectivity. This property of lipase makes it a good catalyst in organic synthesis. Lipases play a vital role in fat digestion and metabolism.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40 °C (triolein, Cat. No. 62314 as substrate)

Other Notes

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.
Chemoenzymatic synthesis of (-)-carbocyclic 7-deazaoxetanocin G

enzyme

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Rudolf Zechner et al.
Cell metabolism, 15(3), 279-291 (2012-03-13)
Lipolysis is defined as the catabolism of triacylglycerols stored in cellular lipid droplets. Recent discoveries of essential lipolytic enzymes and characterization of numerous regulatory proteins and mechanisms have fundamentally changed our perception of lipolysis and its impact on cellular metabolism.
Biochemistry, Lipase
Pirahanchi Y and Sharma S
StatPearls [Internet] (2022)
X. Chen et al.
Tetrahedron Letters, 33, 2249-2249 (1992)
Emily Cottam et al.
Medical engineering & physics, 31(2), 221-226 (2008-09-02)
This paper investigates the effect of sterilisation by gamma irradiation (dose 2.5Mrad) on the following properties of polycaprolactone (PCL): (1) degradation rate (catalysed by lipase), (2) mechanical properties, (3) the ability of cells to attach and subsequently grow on its
P Berglund
Biomolecular engineering, 18(1), 13-22 (2001-06-29)
Lipases are used frequently as chiral catalysts in the synthesis of various fine chemicals and intermediates. The increasing need of compounds with high stereochemical purity requires catalysts with an improved and controlled performance. This overview emphasizes some important aspects for
View All Related Papers

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