62639
Lowicryl® K4M reagent kit
Synonym(s):
HMDSO
impurities
methacrylates (not stabilized)
suitability
suitable for microscopy
application(s)
hematology
histology
storage temp.
2-8°C
SMILES string
OC(=O)c1ccc2ccc3cccc4ccc1c2c34
Application
Polar, methacrylate/acrylate based, low- temperature embedding resin for biological material. Can be used with either the progressive lowering of temperature technique or freeze substitution.; Applicable temperature range, 220K to 340K
Legal Information
Lowicryl is a registered trademark of Polysciences
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Skin Corr. 1B
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
194.0 °F - closed cup
Flash Point(C)
90 °C - closed cup
Regulatory Information
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L G Altman et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 32(11), 1217-1223 (1984-11-01)
Lowicryl K4M (K4M) was recently introduced as an embedding medium for immunocytochemistry at the electron microscope level (BL Armbruster, E Carlemalm, R Chiovetti, RM Garavito, JA Hobot, E Kellenberger, W Villiger (1982):J Microsc 126:77 and E Carlemalm, M Garavito, W
[Use of ultrastructural in situ hybridization for the intracellular detection of viral DNA and RNA].
F Puvion-Dutilleul
Pathologie-biologie, 41(2), 208-212 (1993-02-01)
Post-embedding in situ nucleic acid hybridization (ISH) at the electron microscope level is at present the best tool for the ultrastructural detection of specific viral sequences in infected cells since sensitivity and specificity of the method are paralleled by preservation
S Noda et al.
Journal of nutritional science and vitaminology, 39(2), 201-205 (1993-04-01)
The localization of rat small intestinal microvillous sucrase-isomaltase was studied by immunoelectron microscopy to investigate its intracellular transport to the microvillous membrane. At the same time, the usefulness of a rapid embedding method of tissues in Lowicryl K4M for immunocytochemistry
J J Bogers et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 44(1), 43-48 (1996-01-01)
In this study we compared the recently commercialized electron microscopy embedding resin Unicryl with the well-known resin Lowicryl K4M with regard to morphological and immunohistochemical preservation properties. The standard embedding procedure recommended by the manufacturer for the use of Unicryl
H B Chen et al.
Current eye research, 17(6), 642-649 (1998-07-15)
To investigate the histochemistry of the tear film, conjunctival goblet cells and corneal superficial epithelial cells by an in vivo cryofixation with the freeze-substitution method. Following rapid freezing of the eyeballs by an isopentane-propane mixture cooled by liquid nitrogen directly
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