62639
Lowicryl® K4M reagent kit
Synonym(s):
HMDSO
SMILES string
OC(=O)c1ccc2ccc3cccc4ccc1c2c34
impurities
methacrylates (not stabilized)
suitability
suitable for microscopy
application(s)
hematology
histology
storage temp.
2-8°C
Application
Polar, methacrylate/acrylate based, low- temperature embedding resin for biological material. Can be used with either the progressive lowering of temperature technique or freeze substitution.; Applicable temperature range, 220K to 340K
Legal Information
Lowicryl is a registered trademark of Polysciences
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Skin Corr. 1B
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
194.0 °F - closed cup
flash_point_c
90 °C - closed cup
Regulatory Information
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B G Schneider et al.
Investigative ophthalmology & visual science, 27(5), 679-688 (1986-05-01)
Interstitial retinol binding protein (IRBP) is a soluble glycoprotein found in the interphotoreceptor matrix (IPM) and implicated in shuttling retinol between retina and pigment epithelium (PE) cells. The authors have studied the distribution of IRBP by EM immunocytochemistry. Thin sections
L G Altman et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 32(11), 1217-1223 (1984-11-01)
Lowicryl K4M (K4M) was recently introduced as an embedding medium for immunocytochemistry at the electron microscope level (BL Armbruster, E Carlemalm, R Chiovetti, RM Garavito, JA Hobot, E Kellenberger, W Villiger (1982):J Microsc 126:77 and E Carlemalm, M Garavito, W
[Use of ultrastructural in situ hybridization for the intracellular detection of viral DNA and RNA].
F Puvion-Dutilleul
Pathologie-biologie, 41(2), 208-212 (1993-02-01)
Post-embedding in situ nucleic acid hybridization (ISH) at the electron microscope level is at present the best tool for the ultrastructural detection of specific viral sequences in infected cells since sensitivity and specificity of the method are paralleled by preservation
J J Bogers et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 44(1), 43-48 (1996-01-01)
In this study we compared the recently commercialized electron microscopy embedding resin Unicryl with the well-known resin Lowicryl K4M with regard to morphological and immunohistochemical preservation properties. The standard embedding procedure recommended by the manufacturer for the use of Unicryl
S Noda et al.
Journal of nutritional science and vitaminology, 39(2), 201-205 (1993-04-01)
The localization of rat small intestinal microvillous sucrase-isomaltase was studied by immunoelectron microscopy to investigate its intracellular transport to the microvillous membrane. At the same time, the usefulness of a rapid embedding method of tissues in Lowicryl K4M for immunocytochemistry
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