SIGi001-A-13

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Depositor
Sigma-Aldrich

Derivation
Primary cell type: -
Reprogramming method
Vector type: None

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4) Yes
TRA 1-60 Yes
SSEA-3 Yes
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -

Genotyping
Karyotyping
Karyotyping method: G-Banding

Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimer′s disease
Type of modification: Isogenic
Gene: MAPT
Chromosome location: 17q21.31
Zygosity: Heterozygous
Target locus modification: Mutated
Description: MAPT 10+16T Mono allelic
Disease: Frontotemporal dementia

Note: EAUA and CLIP must be completed before order fulfillment

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