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Merck
CN

67351

Atto 620 NHS ester

BioReagent, suitable for fluorescence, ≥80% (coupling to amines)

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Technical Service
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product line

BioReagent

assay

≥80% (coupling to amines)

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm, >90%, 620 nm, >90%

fluorescence

λex 620 nm; λem 641 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 620 NHS ester Atto 540 Q NHS ester is used to label molecules such as proteins via conjugation to primary amine groups.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Simone Nicolardi et al.
Analytical chemistry, 84(5), 2512-2520 (2012-02-11)
Fluorescent protein labeling has been an indispensable tool in many applications of biochemical, biophysical, and cell biological research. Although detailed information about the labeling stoichiometry and exact location of the label is often not necessary, for other purposes, this information
Maria Strianese et al.
Inorganic chemistry, 51(21), 11220-11222 (2012-10-18)
Fluorescently labeled cobalt peptide deformylase (Co-PDF) can be efficiently used as a fluorescence-resonance-energy-transfer-based sensing device for hydrogen sulfide (H(2)S). The proof of concept of our sensor system is substantiated by spectroscopic, structural, and theoretical results. Monohydrogen sulfide coordination to Co-PDF
Maria Strianese et al.
Journal of inorganic biochemistry, 104(6), 619-624 (2010-03-23)
In this paper we explore the use of fluorescently labeled cytochrome c peroxidase (CcP) from baker's yeast for monitoring nitric oxide (NO) down to the sub-micromolar level, by means of a FRET (Förster Resonance Energy Transfer) mechanism. The binding affinity



Global Trade Item Number

SKUGTIN
67351-1MG-F04061823354974