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Merck
CN

68998

Sigma-Aldrich

Esterase Isoenzyme 4 porcine liver, recombinant

recombinant, expressed in E. coli, ≥0.7 U/mg

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About This Item

CAS Number:
EC Number:
UNSPSC Code:
12352204
NACRES:
NA.54
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recombinant

expressed in E. coli

Quality Level

form

crystalline
crystals
powder or flakes

specific activity

≥0.7 U/mg

storage temp.

−20°C

Application

Esterase Isoenzymes may be used to identify the monocytic element in normal and leukemic cells. They may be used for the biochemical characterization of different hematopoietic cell lineages and stages of differentiation when studying leukemias and lymphomas. Esterase Isoenzyme 4, product 68998, is recombinant and from porcine liver. It is expressed in E. coli.

Biochem/physiol Actions

An esterase is a hydrolase that splits esters into acids and alcohols.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

1 U corresponds to the amount of enzyme which converts 1 μmol 4-nitrophenyl-L-acetate per minute at pH 7.5 and 30°C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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H G Drexler et al.
Cancer, 59(1), 77-82 (1987-01-01)
The expression of a particular alpha-naphthyl acetate esterase isoenzyme which is specific for monocytes was examined in a panel of cultured leukemia-lymphoma cell lines (n = 88), freshly obtained leukemia-lymphoma cells (n = 527), and in fresh (n = 10)
Esterase isoenzyme profiles of 255 leukemia-lymphoma cell lines from all hematopoietic cell lineages.
SM Gignac, ZB Hu, et al.
Leukemia Lymphoma, 22, 143-151 (1996)
Ian Cushman et al.
The Journal of biological chemistry, 288(26), 19177-19183 (2013-05-10)
A number of proteins that play key roles in cell signaling are post-translationally modified by the prenylation pathway. The final step in this pathway is methylation of the carboxyl terminus of the prenylated protein by isoprenylcysteine carboxylmethyltransferase. Due to the
Colin J Jackson et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(25), 10177-10182 (2013-06-05)
Insect carboxylesterases from the αEsterase gene cluster, such as αE7 (also known as E3) from the Australian sheep blowfly Lucilia cuprina (LcαE7), play an important physiological role in lipid metabolism and are implicated in the detoxification of organophosphate (OP) insecticides.
Yingling Wei et al.
Chemico-biological interactions, 204(2), 75-79 (2013-05-07)
We have recently synthesized a series of phosphorylated flavonoids and identified some of them as potent inhibitors of pancreatic cholesterol esterase (CEase) with excellent selectivity for CEase over acetylcholinesterase (AChE). In the present paper, we investigated the inhibitory activities of

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