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Merck
CN

77636

Phenolphthalein β-D-glucuronide

≥98% (TLC)

Synonym(s):

Phenolphthalein β-D-glucosiduronic acid, Phenolphthalein glucuronic acid, Phenolphthalein mono-β-D-glucosiduronic acid

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About This Item

Empirical Formula (Hill Notation):
C26H22O10
CAS Number:
Molecular Weight:
494.45
UNSPSC Code:
12352204
NACRES:
NA.83
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
68526
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InChI

1S/C26H22O10/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32/h1-12,19-22,25,27-30H,(H,31,32)/t19-,20-,21+,22-,25+,26?/m0/s1

SMILES string

O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)C3(OC(=O)c4ccccc34)c5ccc(O)cc5

InChI key

FXJYOZKDDSONLX-XADSOVDISA-N

biological source

rabbit urine

assay

≥98% (TLC)

form

powder

impurities

≤0.5% free phenolphthalein, ≤6% water

solubility

H2O: 0.1 g/mL, clear, light yellow

storage temp.

−20°C

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Application

β-glucuronidase test: suitable as substrate, for liver and bacterial enzymes

Other Notes

Characterization of β-glucuronidase from Ampullaria

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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T. Tsukada et al.
Comparative Biochemistry and Physiology, 86B, 565-565 (1987)
Adam G Staines et al.
Analytical biochemistry, 342(1), 45-52 (2005-06-17)
A primary reason for poor characterization of microsomal transport to date is the limitations of the measurement techniques used. Radiodetection provides sufficient sensitivity, but it can be applied only when labeled analogue is available. In this article, we report the
Takahiro Sasamoto et al.
Journal of gastroenterology and hepatology, 21(1 Pt 1), 26-31 (2006-05-19)
There are several reports on the biliary excretion of bile acids and organic anions in zone 1- and zone 3-injured rat liver, but the results are controversial. In order to dissolve the discrepancy between previous works about the role of
M Csala et al.
Biochemical pharmacology, 59(7), 801-805 (2000-03-16)
The physiological function of microsomal beta-glucuronidase is unclear. Substrates may be either glucuronides produced in the lumen of endoplasmic reticulum (ER) or those taken up by hepatocytes. In the latter case, efficient inward transport of glucuronides at the plasma membrane
J A Wilhelm et al.
Journal of chromatography, 578(2), 231-238 (1992-07-24)
A procedure is described to simultaneously quantitate phenolphthalein and its glucuronide metabolite from dog serum, urine and bile using high-performance liquid chromatography. The major advantages of this over pre-existing methods include direct analysis of the parent compound and glucuronide metabolite

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