79385
Phosphatase, Alkaline from bovine intestinal mucosa
solution (clear), >10000 U/mL
Synonym(s):
Alkaline Phosphatase from calf intestinal mucosa
form
solution (clear)
specific activity
≥1000 U/mg protein
mol wt
Mr ~140000
concentration
>10000 U/mL
density
1.12 g/mL at 20 °C
storage temp.
2-8°C
Application
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
Physical form
solution in 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 30 mM triethanolamine, pH 7.6
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37°C
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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American journal of human genetics, 92(4), 584-589 (2013-04-09)
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Gouri Chaudhuri et al.
Indian journal of biochemistry & biophysics, 50(1), 64-71 (2013-04-27)
The hydrolysis of p-nitrophenyl phosphate (pNPP) by calf intestinal alkaline phosphatase (CIAP) was investigated with respect to kinetic parameters such as V(max), K(m) and K(cat) under varying pH, buffers, substrate concentration, temperature and period of incubation. Highest activity was obtained
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