79385
Phosphatase, Alkaline from bovine intestinal mucosa
solution (clear), >10000 U/mL
Synonym(s):
Alkaline Phosphatase from calf intestinal mucosa
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
MDL number:
form
solution (clear)
specific activity
≥1000 U/mg protein
mol wt
Mr ~140000
concentration
>10000 U/mL
density
1.12 g/mL at 20 °C
storage temp.
2-8°C
Application
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
Physical form
solution in 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 30 mM triethanolamine, pH 7.6
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37°C
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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