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Merck
CN

83263

BICINE buffer Solution

BioUltra, 1 M in H2O

Synonym(s):

N,N-Bis(2-hydroxyethyl)glycine

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About This Item

Empirical Formula (Hill Notation):
C6H13NO4
CAS Number:
Molecular Weight:
163.17
UNSPSC Code:
12161700
eCl@ss:
32129211
PubChem Substance ID:
Beilstein/REAXYS Number:
1769362
MDL number:
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SMILES string

OCCN(CCO)CC(O)=O

grade

Molecular Biology

product line

BioUltra

concentration

1 M in H2O

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

5.0±0.2

density

1.05 g/mL at 20 °C

λ

neat

UV absorption

λ: 260 nm Amax: <0.07, λ: 280 nm Amax: <0.04

suitability

in accordance for filter test

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

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S Ito et al.
Clinica chimica acta; international journal of clinical chemistry, 115(2), 135-144 (1981-09-10)
Serum guanase activity has been considered as a possible specific indicator of hepatocellular diseases. However, no suitable method is available for routine clinical determination of serum guanase activity. Conventional assay methods are troublesome and inaccurate, since guanine and 8-azaguanine, the
E Gopinath et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(9), 3041-3044 (1989-05-01)
The nature and rate of reduction of Hg2+ to Hg0 by 1,5-dihydro-3,(3-sulfopropyl)lumiflavin (FIH2) in buffered aqueous solutions (pH 4.7) is dependent on the ligation of Hg2+. In the presence of N,N-bis(2-hydroxyethyl)glycine or when ligated to ethylenediaminetetraacetic acid, the reduction is
The myosin dimer: an intermediate in the self-assembly of the thick filament of vertebrate skeletal muscle.
J S Davis et al.
FEBS letters, 140(2), 293-297 (1982-04-19)
Yoshiteru Seo et al.
Magnetic resonance in medicine, 65(4), 1005-1012 (2011-03-18)
The toxicity of free Mn(2+) is a bottleneck for the in vivo application of manganese ion enhanced MRI. To reduce free Mn(2+) concentration ([Mn(2+) ]), a low affinity chelate reagent: N,N-bis(2-hydroxyethyl)glycine (bicine) was used. Considering the conditional association constant of
R N Roy et al.
Cryobiology, 21(6), 672-681 (1984-12-01)
The establishment of the pH (designated pH*) of a standard buffer solution suitable as a pH reference in 30, 40, and 50 mass% dimethyl sulfoxide (DMSO)/H2O mixtures at temperatures in the range -20 to 0 degrees C is reported. The

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