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90182

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride

≥99.0% (AT)

Synonym(s):

(3S)-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride, (3S)-7-Amino-1-chloro-3-tosylamino-2-heptanone hydrochloride, TLCK, Tosyl-L-lysyl-chloromethane hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C14H21ClN2O3S · HCl
CAS Number:
4272-74-6
Molecular Weight:
369.31
MDL number:
MFCD00065395
Beilstein/REAXYS Number:
7106867
NACRES:
NA.77
PubChem Substance ID:
57653369
eCl@ss:
32160406
UNSPSC Code:
12352202
EC Number:
224-266-4
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biological source

synthetic

Quality Level

100

assay

≥99.0% (AT)

form

powder or crystals

optical activity

[α]20/D −7.8±0.5°, c = 2% in H2O

storage temp.

−20°C

SMILES string

Cl[H].Cc1ccc(cc1)S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl

InChI

1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1

InChI key

YFCUZWYIPBUQBD-ZOWNYOTGSA-N

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Other Notes

An alkylating reagent that may react with histidine or cysteine residues at the active site of enzymes. Inactivates serine proteases such as trypsin and clostripain . Chymotrypsin is not affected. To prevent proteolytic degradation, a combination of TLCK and TPCK can be used throughout the isolation of proteins, as in the isolation of histones.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1B

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL8593
BCCL6102
BCCL6681
BCCK7268
BCCL1911

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J M Griscavage et al.
Biochemical and biophysical research communications, 215(2), 721-729 (1995-10-13)
The objective of this study was to ascertain the mechanism by which serine and cysteine proteinase inhibitors interfere with production of NO by LPS-activated rat alveolar macrophages. Macrophages were incubated in the presence of LPS+ test agent for 24 hr.
Further observations on substrate-derived chloromethyl ketones that inactivate trypsin.
E Shaw et al.
Archives of biochemistry and biophysics, 139(2), 298-305 (1970-08-01)
Victor F Zevallos et al.
European journal of nutrition, 58(4), 1507-1514 (2018-03-31)
Wheat amylase trypsin inhibitors (ATI) are dietary non-gluten proteins that activate the toll-like receptor 4 on myeloid cells, promoting intestinal inflammation. We investigated the effects of dietary ATI on experimental allergic airway inflammation. Mice on a gluten and ATI-free diet
Studies on the active site of clostripain. The specific inactivation by the chloromethyl ketone derived from -N-tosyl-L-lysine.
W H Porter et al.
The Journal of biological chemistry, 246(24), 7675-7682 (1971-12-25)
I Kourteva et al.
Analytical biochemistry, 162(2), 345-349 (1987-05-01)
A technique for quickly detecting nanogram quantities of low- and high-molecular-weight inhibitors of some serine proteases is described. The inhibitor solutions are spotted onto agar films which contain either L-1-p-tosylamino-2-phenylethyl chloromethyl ketone (TPCK)-trypsin or tosyl lysine chloromethyl ketone (TLCK)-chymotrypsin. Enzyme
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Global Trade Item Number

SKUGTIN
90182-100MG04061833246443
90182-500MG04061833267325

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