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Merck
CN

92544

Abberior® FLIP 565, maleimide

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352111
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Quality Segment

form

solid

concentration

≥50.0% (degree of coupling)

solubility

DMF: 0.25 mg/mL, clear

fluorescence

λex 565 nm; λem 580 nm±5 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Absorption Maximum (off-state) λmax:314 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 47,000 M-1cm-1 (MeOH)
Fluorescence Maximum, λfl:580 nm (PBS, pH 7.4)
Photoactication Wavelength: 310-380 (one-photon activation)
650-800 (two-photon activation)
Fluorescence Quantum Yield, η: 0.38 (PBS, pH 7.4)

Application

Abberior® FLIP 565 conjugated with secondary antibody has been used for STORM (stochastic optical reconstruction microscopy) imaging of COS-7 and S180 cells.
Designed and tested for fluorescent super-resolution microscopy

Legal Information

abberior is a registered trademark of Abberior GmbH


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Remi Galland et al.
Nature methods, 12(7), 641-644 (2015-05-12)
Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported



Global Trade Item Number

SKUGTIN
92544-1MG04061823360227