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Merck
CN

93356

Tricine

BioUltra, ≥99.5% (NT)

Synonym(s):

N-[Tris(hydroxymethyl)methyl]glycine

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About This Item

Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
Molecular Weight:
179.17
EC Number:
227-193-6
UNSPSC Code:
12161700
PubChem Substance ID:
Beilstein/REAXYS Number:
1937804
MDL number:
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product line

BioUltra

assay

≥99.5% (NT)

impurities

Insoluble matter, passes filter test

ign. residue

≤0.05% (as SO4)

loss

≤0.5% loss on drying, 110 °C

pH

4.5-6.0 (25 °C, 0.5 M in H2O)

useful pH range

7.4-8.8

pKa (25 °C)

8.1

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤500 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.03, λ: 280 nm Amax: 0.02

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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Application

Buffer component for separation of low molecular weight peptides.

Biochem/physiol Actions

Tricine, a buffer with a useful range from 7.4 to 8.8, is commonly used for the electrophoretic separation of low molecular weight proteins in gels and membranes. It is an effective buffer for ATP assays using firefly luciferase. Tricine is also an effective hydroxyl radical scavenger used to protect molecules during radical generating procedures such as irradiation.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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J.J. Webster et al.
Journal of Applied Biochemistry, 2, 469-469 (1980)
J Ambler et al.
Clinical chemistry, 26(8), 1221-1223 (1980-07-01)
Two new non-barbiturate buffers have been formulated for serum protein electrophoresis on cellulose acetate membranes. Both buffers give five distrinct fractions and are suitable for all systems, but have been primarily designed to meet the needs of the Gelman "Sepratek"
Thierry Rabilloud
Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
Hermann Schägger
Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in

Global Trade Item Number

SKUGTIN
H24003-10G04061833792100
54826-50MG04061834245858
H24003-100G04061833792063
H24003-25G04061833792124
93979-100G04061833379158
93979-25G04061833415146

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