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Merck
CN

93426

Triton™ X-100

BioXtra

Synonym(s):

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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About This Item

Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
9036-19-5
PubChem Substance ID:
329770232
UNSPSC Code:
12352200
Beilstein/REAXYS Number:
2315025
MDL number:
MFCD00128254

Key Documents

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grade

Molecular Biology

description

non-ionic

product line

BioXtra

form

liquid

mol wt

micellar avg mol wt 80,000, average mol wt 625

aggregation number

100-155

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, permeability assay: suitable

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

ign. residue

≤0.2%

CMC

0.2-0.9 mM (20-25°C)

transition temp

cloud point 65 °C, pour point ~7 °C

solubility

H2O: 0.005 M at 20 °C, clear, colorless

density

1.065 g/mL at 20 °C

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤500 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤500 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

HLB

13.5

storage temp.

2-8°C

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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General description

Triton™ X-100 is a non-ionic surfactant that aids the recovery of membrane-bound proteins under mild non-denaturing conditions.

Application

Suitable for extraction of membrane-bound proteins

Biochem/physiol Actions

Triton™ X-100 compromises the integrity of the lipid bilayer by disrupting the polar head group on the hydrogen bonding. The detergent monomer inserts itself into the lipid bilayer resulting in over-permeabilization and disruption of the cell structure.
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBH2984V
BCBD2755V
BCBB4485V
BCBC7803
BCBB4485

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Microinjection into Xenopus oocytes: equipment.
M J Hitchcock et al.
Methods in enzymology, 152, 276-284 (1987-01-01)
Dipankar Koley et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(39), 16783-16787 (2010-09-15)
Changes in HeLa cell morphology, membrane permeability, and viability caused by the presence of Triton X-100 (TX100), a nonionic surfactant, were studied by scanning electrochemical microscopy (SECM). No change in membrane permeability was found at concentrations of 0.15 mM or
Wentao Li et al.
Virus research, 235, 6-13 (2017-04-02)
Porcine epidemic diarrhea virus (PEDV) is an emerging pathogenic coronavirus that causes a significant economic burden to the swine industry. The virus infects the intestinal epithelium and causes villous atrophy, resulting in diarrhea and dehydration. Interaction of the viral spike
Friederike Pfeiffer et al.
Acta neuropathologica, 122(5), 601-614 (2011-10-11)
In experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis (MS), loss of the blood-brain barrier (BBB) tight junction (TJ) protein claudin-3 correlates with immune cell infiltration into the CNS and BBB leakiness. Here we show that sealing BBB
Jing Cui et al.
Experimental eye research, 88(3), 438-444 (2008-11-27)
Previous investigators reported that epiretinal membranes isolated from patients with proliferative vitreoretinopathy (PVR) express various platelet-derived growth factor (PDGF) family members and PDGF receptors (PDGFRs) (Cui, J.Z., Chiu, A., Maberley, D., Ma, P., Samad, A., Matsubara, J.A., 2007. Stage specificity
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