Urease from Canavalia ensiformis (Jack bean)

Name: Urease from <I>Canavalia ensiformis</I> (Jack bean)
Brand: SIGMA

powder, ~1 U/mg

Synonym(s):

Jack bean urease, Urea amidohydrolase

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About This Item

CAS Number:

9002-13-5

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

232-656-0

NACRES:

NA.54

MDL number:

MFCD00070858

EC Number:

3.5.1.5 (BRENDA, IUBMB)

Specific activity:

~1 U/mg

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Properties

form

powder

Quality Segment

100

specific activity

~1 U/mg

mol wt

M_r ~480000

storage temp.

−20°C

Description

General description

Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease consists of nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Application

Urease from Canavalia ensiformis (Jack bean) has been used in purification and crystallization studies.

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .