C4482
CelLytic™ Y Cell Lysis Reagent
For yeast cells
Synonym(s):
Yeast cell lysis reagent
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About This Item
Quality Level
form
solution
Related Categories
General description
Application
Features and Benefits
- Fast: Cell lysis in just 20 minutes
- Efficient: Three times more efficient than glass beads
- Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
- Gentle: Non-denaturing - preserves biological activity
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Regulatory Information
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Can I use glass beads with CelLytic™ Y Cell Lysis Reagent, Product C4482?
If cell growth conditions are not optimal, then glass beads can be used to increase yields.
Should I use CelLytic™ Y Cell Lysis Reagent, Product C4482?
For all cases in which the addition of DTT does not interfere with the application tested, it is recommended to add DTT (5 - 10 mM final concentration) to the CelLytic Y reagent. Addition of DTT significantly improves total protein yield.
Can I use CelLytic™ Y Cell Lysis Reagent, Product C4482, to make spheroplasts?
No. For spheroplast formation and protein extraction we recommend the CelLytic Y Plus kit.
What is in CelLytic™ Y Cell Lysis Reagent, Product C4482?
The formulation is considered proprietary, but we can state that it is a non-denaturing phosphate-free reagent.
What strains have been used with CelLytic™ Y Cell Lysis Reagent, Product C4482?
The efficiency of CelLytic Y reagent for protein extraction has been tested on different strains of Saccharomyces cerevisiae [Y187, Y190, W303(a), S288C, SP1, S1278b (diploid and haploid), BJ2168, and cdc25] and Schizosaccharomyces pombe.
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Articles
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.
CelLytic Y offers phosphate-free yeast cell lysis, while CelLytic Y Enzymatic Kit ensures efficient protein extraction from various yeast strains.
我们的酵母细胞裂解试剂具有通用性、无磷酸盐、非变性等特点。CelLytic Y细胞裂解试剂可作为酵母细胞裂解和蛋白溶解的有效方法。CelLytic Y酵母酶解试剂盒为从酿酒酵母、巴斯德毕赤酵母和粟酒裂殖酵母酵母菌株中高效形成原生质球和提取蛋白提供了一种便捷的方法。
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细胞裂解和蛋白质溶解是进行有效分析和高效处理的关键步骤，默克分享提取方法包括酶法、化学法、机械法或几种方法的联合使用。
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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