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E8156

Anti-eRF1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Eukaryotic translation termination factor 1, Anti-RF1, Anti-Release factor 1, Anti-eTF1

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
7
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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 1-2 μg using HeLa cell lysates., western blot: 0.5-1 μg/mL using 3T3 or Rat1 cell extracts.

UniProt accession no.

P62495

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ETF1(2107)
mouse ... Etf1(225363)
rat ... Etf1(307503)

General description

Eukaryotic translation termination factor 1 (eRF1) is a protein encoded by the ETF1 gene in humans. It belongs to eRF1 family and the amino-acid sequence is highly conserved.

Application

Anti-eRF1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Biochem/physiol Actions

Eukaryotic translation termination factor 1 (eRF1) proteins are directly implicated in the termination of translation in eukaryotes. It plays an important role in the termination of protein synthesis, which is performed by three domains (N, M and C) of eRF1. Rearrangement in eRF1 leads to N-domain accommodation into the ribosomal A site.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

低风险生物材料
常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
0000145856
0000242787
0000242787
0000106154

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Related Content

Data Sheet - E8156

Instructions

Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division
Petsch K, et al.
Plant Physiology, 139(1), 115-126 (2005)
L Frolova et al.
Nature, 372(6507), 701-703 (1994-12-15)
The termination of protein synthesis in ribosomes is governed by termination (stop) codons in messenger RNAs and by polypeptide chain release factors (RFs). Although the primary structure of prokaryotic RFs and yeast mitochrondrial RF is established, that of the only
Toshiaki Shigeoka et al.
Nucleic acids research, 40(14), 6887-6897 (2012-05-05)
Upf1 is a highly conserved RNA helicase essential for nonsense-mediated mRNA decay (NMD), an mRNA quality-control mechanism that degrades aberrant mRNAs harboring premature termination codons (PTCs). For the activation of NMD, UPF1 interacts first with a translation-terminating ribosome and then
View All Related Papers

Global Trade Item Number

SKUGTIN
E8156-200UL04061837771330