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Merck
CN

F7378

Fluorescein diacetate

Powder

Synonym(s):

3,6-Diacetoxyfluoran, Di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):
C24H16O7
CAS Number:
596-09-8
Molecular Weight:
416.38
NACRES:
NA.47
PubChem Substance ID:
24894939
UNSPSC Code:
12171500
EC Number:
209-877-6
MDL number:
MFCD00005062
Beilstein/REAXYS Number:
62575

Key Documents

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Product Name

Fluorescein diacetate, used as cell viability stain

Quality Level

200

form

powder

technique(s)

titration: suitable

color

white to faint yellow

mp

200-203 °C (lit.)

solubility

acetone: 25 mg/mL

ε (extinction coefficient)

≥86000 at 488-494 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

InChI key

CHADEQDQBURGHL-UHFFFAOYSA-N

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General description

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.
Lipase substrate

Application

Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT2237
0000460551
0000460551
0000413679
0000413679

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The influence of scaffold microstructure on chondrogenic differentiation of mesenchymal stem cells.
Zhang J
Biomedical Materials (Bristol, England), 9, 035011-035011 (2014)
Effect of shock waves on suspended and immobilized L1210 cells.
Brummer F
Ultrasound in Medicine & Biology, 15, 229-229 (1989)
Cell Growth, Differentiation and Senescence: A Practical Approach (1999)
Koichi Baba et al.
Journal of controlled release : official journal of the Controlled Release Society, 153(3), 278-287 (2011-05-10)
This report describes a method for enhancing the ocular penetration of eye drops using nanoparticles of hydrolyzable dye, which is similar to a prodrug approach. The entry of eye drops into the ocular globe is restricted predominantly by corneal barrier
Melina M Malinen et al.
Tissue engineering. Part A, 18(23-24), 2418-2425 (2012-06-21)
Current hepatocyte models do not mimic the human liver morphology and functions properly and, therefore, drug metabolism, excretion, and toxicity in the liver are inadequately predicted. In this study, we established three-dimensional (3D) hepatic cell cultures in hydrogels of peptide
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Global Trade Item Number

SKUGTIN
F7378-100G04061833619599
F7378-25G04061835347735
F7378-10G04061835347728
F7378-5G04061835347742

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