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Merck
CN

HT501850

Formalin solution, neutral buffered, 10%

histological tissue fixative

Synonym(s):

10% NBF, 10% Neutral Buffered Formalin, Formaldehyde, Methanal

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About This Item

UNSPSC Code:
12171500
PubChem Substance ID:
24895892
NACRES:
NA.47
MDL number:
MFCD00003274

Key Documents

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biological source

synthetic

Quality Level

200

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

pH

6.90-7.10

density

1.080 g/cm3

application(s)

hematology
histology

storage temp.

room temp

SMILES string

[O-]#[C+H2]

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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General description

10% Neutral Buffered Formalin is the most used fixative throughout the world for light microscopy. Formalin solution is the commercially obtained solution containing 37% w/w (40% w/v) of formaldehyde in water. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Application

Formalin solution (neutral buffered, 10%) has been popularly used as a general-purpose histological fixative to fix tissue samples.
  • It effectively preserves the cellular and tissue morphology, maintaining the structural integrity required for microscopic examination.
  • It may be used as a reagent for the primary fixation of tissues for diagnostic applications of an immunoperoxidase method in hematopathology.
  • It is used for routine histological staining, providing clear and detailed images of cellular structures.
Popular general purpose histological fixative.

Biochem/physiol Actions

Formaldehyde is both a non-coagulant and an additive fixative. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins.

Features and Benefits

  • 10% NBF is well-suited for large throughput laboratories.
  • It requires a relatively short period of fixation.
  • It can be used for the long-term storage of tissue.

Physical form

Neutral buffered solution

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

185.0 °F

flash_point_c

85 °C

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

wgk

WGK 3

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ8273
MKCZ7680
MKCZ6233
MKCZ2854
MKCS5167

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Histological and Histochemical Methods ? Theory and practice, 5th Edition
J. A. Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
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Diagnostic applications of an immunoperoxidase method in hematopathology.
Banks PM.
The Journal of Histochemistry and Cytochemistry, 27(8), 1192-1194 (1979)
Xiaozhen Liang et al.
PLoS pathogens, 7(9), e1002220-e1002220 (2011-09-21)
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Coralie Apfeldorfer et al.
Diagnostic pathology, 3 Suppl 1, S16-S16 (2008-08-05)
Historically, histopathology evaluation is performed by a pathologist generating a qualitative assessment on thin tissue sections on glass slides. In the past decade, there has been a growing interest for tools able to reduce human subjectivity and improve workload. Whole
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Global Trade Item Number

SKUGTIN
HT501850-200L04061838207722

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