HT501850
Formalin solution, neutral buffered, 10%
histological tissue fixative
Synonym(s):
10% NBF, 10% Neutral Buffered Formalin, Formaldehyde, Methanal
biological source
synthetic
Quality Level
concentration
10% formalin (approx. 4% formaldehyde)
color
colorless
pH
6.90-7.10
density
1.080 g/cm3
application(s)
hematology
histology
storage temp.
room temp
SMILES string
[O-]#[C+H2]
InChI
1S/CH2O/c1-2/h1H2
InChI key
WSFSSNUMVMOOMR-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
General description
10% Neutral Buffered Formalin is the most used fixative throughout the world for light microscopy. Formalin solution is the commercially obtained solution containing 37% w/w (40% w/v) of formaldehyde in water. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.
Application
Formalin solution (neutral buffered, 10%) has been popularly used as a general-purpose histological fixative to fix tissue samples.
- It effectively preserves the cellular and tissue morphology, maintaining the structural integrity required for microscopic examination.
- It may be used as a reagent for the primary fixation of tissues for diagnostic applications of an immunoperoxidase method in hematopathology.
- It is used for routine histological staining, providing clear and detailed images of cellular structures.
Popular general purpose histological fixative.
Biochem/physiol Actions
Formaldehyde is both a non-coagulant and an additive fixative. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins.
Features and Benefits
- 10% NBF is well-suited for large throughput laboratories.
- It requires a relatively short period of fixation.
- It can be used for the long-term storage of tissue.
Physical form
Neutral buffered solution
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Histological and Histochemical Methods ? Theory and practice, 5th Edition
J. A. Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
Jaehwan Ahn et al.
Biomaterials, 268, 120599-120599 (2020-12-21)
Non-alcoholic fatty liver disease (NAFLD) has become a global pandemic. However, a pharmacological cure has not been approved for NAFLD treatment. The greatest barriers to the development of new treatments are the ambiguous criteria among the NAFLD stages and the
Diagnostic applications of an immunoperoxidase method in hematopathology.
Banks PM.
The Journal of Histochemistry and Cytochemistry, 27(8), 1192-1194 (1979)
Xiaozhen Liang et al.
PLoS pathogens, 7(9), e1002220-e1002220 (2011-09-21)
Human gammaherpesviruses are associated with the development of lymphoproliferative diseases and B cell lymphomas, particularly in immunosuppressed hosts. Understanding the molecular mechanisms by which human gammaherpesviruses cause disease is hampered by the lack of convenient small animal models to study
Caterina Nardella et al.
Science signaling, 2(55), ra2-ra2 (2009-01-30)
The mammalian target of rapamycin (mTOR) is a crucial effector in a complex signaling network commonly disrupted in cancer. mTOR exerts its multiple functions in the context of two different multiprotein complexes: mTOR complex 1 (mTORC1) and mTOR complex 2
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service