Key Documents

View All Documentation

L7533

LB Broth with agar (Lennox)

Name: LB Broth with agar (Lennox)
Brand: SIGMA

EZMix powder microbial growth medium

Synonym(s):

LB broth

Select a Size

Change View

About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

grade

Molecular Biology

Quality Level

200

sterility

non-sterile

form

powder

composition

Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture
food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

Description

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Features and Benefits

Lennox EZMix powder with agar provides:

A budget-friendly alternative to pre-poured plates
Granulated, dust-free format for safer handling and faster mixing
Convenient small package to eliminate weighing
Easy scale-up using larger package sizes
Standard formulation

Preparation Note

1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Still not finding the right product?

Explore all of our products under LB Broth with agar (Lennox)

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

新产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

Articles

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

细菌转化实验方案

感受态细胞生长及其转化（吸收DNA）的通用实验方案。

Global Trade Item Number

SKU	GTIN
L7533-1KG	04061833965559
L7533-6X500ML	04061833965566