O5500
Osmium tetroxide
Sealed ampule.
Synonym(s):
Osmium(VIII)-oxide, Osmic acid
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About This Item
Linear Formula:
OsO4
CAS Number:
Molecular Weight:
254.23
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
244-058-7
MDL number:
Form:
powder
vapor density
8.8 (vs air)
Quality Level
vapor pressure
7 mmHg ( 20 °C)
form
powder
reaction suitability
reagent type: oxidant
bp
130 °C (lit.)
mp
39.5-41 °C (lit.)
SMILES string
O=[Os](=O)(=O)=O
InChI
1S/4O.Os
InChI key
VUVGYHUDAICLFK-UHFFFAOYSA-N
General description
Osmium tetroxide (OsO4) is a heavy tetrahedral, symmetrical molecule composed of osmium, a third-row transition metal. Osmium tetroxide is both a dye, and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium testroxide to ensure preservation of the lipids.
Application
Osmium tetroxide has been used as a fixative reagent for Brevibacterium mcbrellneri, and curcumin treated human lung cancer cell lines′ observation with a scanning electron microscope. It is also suitable as a fixative for ultrastructural analysis of Toxoplasma gondii′s skeletal muscle cells.
Biochem/physiol Actions
Osmium tetroxide chemically combines with lipids making them insoluble; only 7% of the lipids can be extracted after the fixation. Therefore, the cell membranes comprising phospholipids become black and electron-dense after fixation in osmium tetroxide. It is also used to fix small amount of fat maintained in sections during paraffin processing.
Other Notes
Due to the hazardous nature of osmium tetroxide, it is recommended to use an ampule opener when using this chemical.
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Danger
hcodes
Hazard Classifications
Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Eye Dam. 1 - Skin Corr. 1B
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
Regulatory Information
监管及禁止进口产品
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Yukihiko Sugita et al.
The Journal of general virology, 92(Pt 11), 2485-2493 (2011-07-29)
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Carsten Købler et al.
Analytical and bioanalytical chemistry, 406(16), 3863-3873 (2014-01-23)
Ultrastructural characterisation is important for understanding carbon nanotube (CNT) toxicity and how the CNTs interact with cells and tissues. The standard method for this involves using transmission electron microscopy (TEM). However, in particular, the sample preparation, using a microtome to
Manuel Marx et al.
Nature protocols, 7(2), 394-407 (2012-02-04)
In this report, we describe a reliable protocol for biocytin labeling of neuronal tissue and diaminobenzidine (DAB)-based processing of brain slices. We describe how to embed tissues in different media and how to subsequently histochemically label the tissues for light
Global Trade Item Number
| SKU | GTIN |
|---|---|
| O5500-10X1G | 04061833652909 |
| O5500-1G | 04061834235750 |
| O5500-5G-A | 04061834247975 |
| O5500-100G-A | 04061837381171 |
| O5500-250MG | 04061834235774 |
| O5500-250G-A | 04061835517329 |