PF0100
Protease Fluorescent Detection Kit
high sensitivity assay
Synonym(s):
Protease Assay Kit
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About This Item
NACRES:
NA.26
UNSPSC Code:
12352202
Application
Protease Fluorescent Detection Kit has been used:
- in the detection of proteolytic activity using casein based protease assay
- for testing the extracellular proteolytic activity of fungal strains
- for quantifying mitochondrial protease activity
- for measuring the protease production from soil solution
Detection limit: approx. 50 ng trypsin
Preparation Note
Supplied with sufficient reagents for up to 200 one mL assays.
The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.
The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.
Analysis Note
Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).
Kit Components Only
Product No.
Description
- Incubation buffer 25 mL
- FITC-casein solution 5 mL
- Assay buffer 200 mL
- Protease standard (trypsin) 20 μg
- FITC control 50 mg
- Trichloroacetic acid (TCA) solution 30 mL
signalword
Danger
hcodes
target_organs
Respiratory system
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1 - Skin Sens. 1 - STOT SE 3
Regulatory Information
低风险生物材料
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Protocols
Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.
Articles
Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.
Related Content
Instructions
Dramatic increase in stability and longevity of enzymes attached to monodispersive iron nanoparticles.
Sharma A, et al.
IEEE Transactions on Magnetics, 43, 2418-2420 (2007)
Stimulation of soil organic nitrogen pool: The effect of plant and soil organic matter degrading enzymes
Kieloaho AJ, et al.
Soil Biology and Biochemistry, 96(10), 97-106 (2016)
Céline Adam et al.
PloS one, 7(5), e38138-e38138 (2012-06-14)
Mitochondria have their own ATP-dependent proteases that maintain the functional state of the organelle. All multicellular eukaryotes, including filamentous fungi, possess the same set of mitochondrial proteases, unlike in unicellular yeasts, where ClpXP, one of the two matricial proteases, is
Global Trade Item Number
| SKU | GTIN |
|---|---|
| PF0100-1KT | 04061834413271 |