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Merck
CN

A0156

Sigma-Aldrich

N-(4-Aminobutyl)-N-ethylisoluminol

≥90%, powder

Synonym(s):

4-(N1-Ethyl-4-aminobutylamino)phthalic hydrazide, 6-[N-(4-Aminobutyl)-N-ethylamino]-2,3-dihydro-1,4-phthalazinedione, ABEI

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About This Item

Empirical Formula (Hill Notation):
C14H20N4O2
CAS Number:
66612-29-1
Molecular Weight:
276.33
Beilstein:
920895
MDL number:
MFCD00010559
UNSPSC Code:
12352204
PubChem Substance ID:
24890404
NACRES:
NA.83

Key Documents

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Product Name

N-(4-Aminobutyl)-N-ethylisoluminol, ≥90%

Quality Level

200

Assay

≥90%

form

powder

mp

259-260 °C (lit.)

solubility

glacial acetic acid: 50 mg/mL, clear, colorless to yellow

storage temp.

2-8°C

SMILES string

CCN(CCCCN)c1ccc2C(=O)NNC(=O)c2c1

InChI

1S/C14H20N4O2/c1-2-18(8-4-3-7-15)10-5-6-11-12(9-10)14(20)17-16-13(11)19/h5-6,9H,2-4,7-8,15H2,1H3,(H,16,19)(H,17,20)

InChI key

LEOJISUPFSWNMA-UHFFFAOYSA-N

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Features and Benefits

Efficient chemiluminescent NH2-coupling reagent for detection of a wide variety of proteins down to the picomole range.

Other Notes

Reportedly, the use of this material in chemiluminescent assays has distinct advantages over conventional radioimmunoassay.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF6214V
WXBF5562V
0000281409
0000271167
0000260371

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K E Achyuthan
Journal of bioluminescence and chemiluminescence, 13(1), 1-11 (1998-06-03)
A novel and sensitive chemiluminescent assay is described to quantitate the acyl transfer activities of blood coagulation factor XIIIa or liver transglutaminase using aminobutyl-N-ethylisoluminol as acyl acceptor and N,N-dimethylcasein, human plasma fibrinogen or fibronectin as acyl donors. The method involved
Ying Chai et al.
The Analyst, 136(16), 3244-3251 (2011-06-10)
A novel electrochemiluminescence (ECL) aptasensor for platelet-derived growth factor B chain (PDGF-BB) assay was developed by assembling N-(aminobutyl)-N-ethylisoluminol functionalized gold nanoparticles (ABEI-AuNPs) with aptamers as nanoprobes. In the protocol, the biotinylated aptamer capture probes were first immobilized on a streptavidin
Minli Yang et al.
The Analyst, 127(9), 1267-1271 (2002-10-12)
The electrogenerated chemiluminescence (ECL) behavior of N-(4-aminobutyl)-N-ethylisoluminol (ABEI) was studied and it was found that ABEI could produce emission light when oxidized at a +1.0 V (vs. Ag/AgCl) potential in alkaline solution. The addition of H2O2 markedly improved the ECL
Jialing Chen et al.
Clinica chimica acta; international journal of clinical chemistry, 488, 143-149 (2018-11-11)
Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a novel inflammatory biomarker, which is useful as an adjunct identification tool for cardiovascular disease. However, the important limitation of the conventional enzyme-linked immunosorbent assay (PLAC ELISA) for Lp-PLA2 assay is its relatively low sensitivity
C J Strasburger et al.
Journal of bioluminescence and chemiluminescence, 4(1), 112-118 (1989-07-01)
The tetrameric structure of streptavidin and its exceptionally strong affinity to biotin (Ka = 10(15)M 1) can be exploited to achieve an amplification of the signal in immunoassays. In the approach described here streptavidin (STAV) labelled with aminobutylethyl-isoluminol (ABEI) served
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