A0186
N-Acylhexosamine Oxidase from Pseudomonas sp.
suspension, ≥20 units/mg protein
Synonym(s):
N-Acyl-D-glucosamine: oxygen 1-oxidoreductase
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About This Item
form
suspension
specific activity
≥20 units/mg protein
storage temp.
2-8°C
Biochem/physiol Actions
Enyzme converts N-acetyl-D-glucosamine to N-acetyl-D-glucosaminolactone.
Physical form
Suspension in 80% saturated ammonium sulfate.
Other Notes
One unit will oxidize 1.0 μmole of N-acetyl-D-glucosamine to N-acetyl-D-glucosaminolactone per minute at pH 6.8 at 37 °C.
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S Teshima et al.
Clinical chemistry, 34(11), 2291-2294 (1988-11-01)
This enzymatic method for determination of sialic acid involves use of neuraminidase (EC 3.2.1.18), N-acetylneuraminate lyase (EC 4.1.3.3), acylglucosamine 2-epimerase (EC 5.1.3.8), N-acetylhexosamine oxidase (from Pseudomonas sp.), and peroxidase (EC 1.11.1.7). Because the method does not require pyruvic acid in