A0410
Aminoallyl-dUTP sodium salt
≥85%, lyophilized powder
Synonym(s):
5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP
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About This Item
Empirical Formula (Hill Notation):
C12H20N3O14P3 · xNa+
CAS Number:
Molecular Weight:
523.22 (free acid basis)
MDL number:
UNSPSC Code:
41106305
NACRES:
NA.51
Product Name
Aminoallyl-dUTP sodium salt, ≥85%, lyophilized powder
Quality Level
Assay
≥85%
form
lyophilized powder
shipped in
dry ice
storage temp.
−70°C
SMILES string
[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)\C=C\CN)O)(O[P](=O)(O)O)O.N
InChI key
YWKTUHBZWBRYHX-TYYBGVCCSA-N
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Application
Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
- to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
- for the generation of chromatin immunoprecipitation (ChIP) probes
- in the reverse transcription of RNA for the synthesis of fluorescent labelled probes
Biochem/physiol Actions
Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Lorenzo Berti et al.
Nanotechnology, 20(23), 235101-235101 (2009-05-19)
Functional DNA scaffolds can be defined as DNA-based structures comprising chemical moieties facilitating and guiding the immobilization of additional nanocomponents. Due to the limited reactivity of DNA there is currently a need to develop rapid routes to expand its chemical
Miguel A Matilla et al.
Genome biology, 8(9), R179-R179 (2007-09-06)
Mutualistic interactions less well known than those between rhizobia and legumes are commonly found between plants and bacteria, frequently pseudomonads, which colonize roots and adjacent soil areas (the rhizosphere). A global analysis of Pseudomonas putida genes expressed during their interaction
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Cox W G and Singer V L
Biotechniques, 36(1), 114-122 (2004)
Chromatin immunoprecipitation (ChIP) on chip experiments uncover a widespread distribution of NF-Y binding CCAAT sites outside of core promoters
Testa A, et al.
The Journal of Biological Chemistry, 280(14), 13606-13615 (2005)
Effect of the Colorless non-ripening mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening
Eriksson E M, et al.
Plant Physiology, 136(4), 4184-4197 (2004)
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