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Merck
CN

A0483

Monoclonal Anti-Actin (20-33) antibody produced in rabbit

clone SIG2-AC2, ascites fluid

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
SIG2-AC2, monoclonal
Application:
ELISA (i), ICC, IF, IHC, WB
Citations:
10

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SIG2-AC2, monoclonal

mol wt

antigen ~42 kDa

species reactivity

chicken, rat, human

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, indirect immunofluorescence: 1:400, western blot: 1:50-1:100 using chicken gizzard total extract

isotype

IgG

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)

General description

Monoclonal Anti-Actin (20-33) (rabbit IgG) is a rabbit monoclonal derived from the hybridoma SIG2-AC2 produced by the fusion of rabbit myeloma cells and splenocytes from rabbits immunized with a synthetic peptide. Actin is one of the most conserved eukaryotic proteins and is expressed in mammals and birds with at least six isoforms characterized by electrophoresis and amino acid sequence analysis. There are three α-actins (skeletal, cardiac and smooth muscle), one β-actin (β-non-muscle) and two γ-actins (γ-smooth muscle and g-non-muscle).

Immunogen

synthetic peptide corresponding to amino acids 20-33 of actin, attached to a multiple antigen peptide (MAP) backbone.

Application

Monoclonal Anti-Actin (20-33) antibody produced in rabbit has been used in:
  • western blotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • immunocytochemistry

Physical form

Supplied as ascites fluid containing 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
075M4843V
100M4815
108K4750

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Melania Cruciani et al.
Frontiers in immunology, 10, 2622-2622 (2019-11-30)
Understanding Staphylococcus aureus (S. aureus)-host immune system interaction is crucial to meet the tremendous medical need associated with this life-threatening bacterial infection. Given the crucial role of dendritic cells (DC) in dictating immune responses upon microbial challenge, we investigated how
The Potential Roles of Actin in The Nucleus
Falahzadeh K, et al.
Cell Journal, 17(1), 7-7 (2015)
PP2A blockade inhibits autophagy and causes intraneuronal accumulation of ubiquitinated proteins
Magnaudeix A, et al.
Neurobiology of Aging, 34(3), 770-790 (2013)
Overexpression of MCPH1 inhibits uncontrolled cell growth by promoting cell apoptosis and arresting the cell cycle in S and G2/M phase in lung cancer cells
Zhou L, et al.
Oncology Letters, 11(1), 365-372 (2016)
Limin Zhou et al.
Oncology letters, 11(1), 365-372 (2016-02-13)
Microcephalin (MCPH1/BRIT1) is a large nuclear protein that is involved in the early cellular response to DNA damage, the expression of which is reduced in a variety of types of human tumors. A recent study by our group demonstrated that
View All Related Papers

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Data Sheet - A0483

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