A0802
Adipic acid dihydrazide–Agarose
saline suspension
Synonym(s):
Adipic acid dihydrazide agarose beads
biological source
plant
Quality Level
form
saline suspension
extent of labeling
≥8 μmol per mL
technique(s)
microbe id | metabolite detection: suitable
matrix activation
cyanogen bromide
matrix spacer
11 atoms (when ligands (aldehydes, carboxylic acids) are coupled through free hydrazide groups)
suitability
suitable for chromatography
storage temp.
2-8°C
Application
Adipic acid dihydrazide Agarose can be used in proteomics and protein chromatography. It has been utilized in research for purifying and identifying the fungal phytotoxin Fusicoccin (FC), identifying RNA binding proteins that interact with RNA cis-elements, and enzyme purification such as peroxisomes from guinea pig liver.
Physical form
Suspension in 0.5 M NaCl containing preservative.
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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A H de Boer et al.
Plant physiology, 89, 250-259 (1989-01-01)
Fusicoccin (FC), a fungal phytotoxin, stimulates the H(+) -ATPase located in the plasma membrane (PM) of higher plants. The first event in the reaction chain leading to enhanced H(+) -efflux seems to be the binding of FC to a FC-binding
Ruben Hovhannisyan et al.
BioTechniques, 46(2), 95-98 (2009-03-26)
Use of RNA affinity chromatography is commonly used to identify RNA binding proteins that interact with specific RNA cis-elements that function in post-transcriptional gene regulation. These purifications can be complicated by residual RNase activity in cellular extracts that can degrade
D Grate et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(11), 6131-6136 (1999-05-26)
The biological function of specific gene products often is determined experimentally by blocking their expression in an organism and observing the resulting phenotype. Chromophore-assisted laser inactivation using malachite green (MG)-tagged antibodies makes it possible to inactivate target proteins in a
Alice Tianbu Zhang et al.
Journal of cell science, 124(Pt 12), 2058-2069 (2011-05-26)
Non-coding Y RNAs are required for the initiation of chromosomal DNA replication in mammalian cells. It is unknown how they perform this function or if they associate with a nuclear structure during DNA replication. Here, we investigate the association of
M Caputi et al.
The EMBO journal, 18(14), 4060-4067 (1999-07-16)
Splicing of the human immunodeficiency virus type 1 (HIV-1) pre-mRNA must be inefficient to provide a pool of unspliced messages which encode viral proteins and serve as genomes for new virions. Negative cis-regulatory elements (exonic splicing silencers or ESSs) are
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