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A1043

Anti-Chicken IgY (IgG) (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

Name: Anti-Chicken IgY (IgG) (whole molecule)−Alkaline Phosphatase antibody produced in rabbit
Brand: SIGMA

IgG fraction of antiserum, buffered aqueous solution

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About This Item

UNSPSC Code:

12352203

MDL number:

MFCD00162223

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biological source

rabbit

conjugate

alkaline phosphatase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:15,000, dot blot: 1:60,000-1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, western blot (chemiluminescent): 1:80,000

shipped in

wet ice

storage temp.

2-8°C

Application

Rabbit anti-chicken IgG alkaline phosphatase conjugated was used for western blot analysis of chicken protein lysates at a dilution of 1:1000 for 1 hour shaking at room temperature. Rabbit anti-chicken IgG alkaline phosphatase conjugated was used for western blot analysis of crude supernatant from A. castellanii trophozoites cultures at a 1:2000 dilution for 1 hour in 5% nofat dry milk.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Pathogenic Acanthamoeba spp secrete a mannose-induced cytolytic protein that correlates with the ability to cause disease.

Michael Hurt et al.

Infection and immunity, 71(11), 6243-6255 (2003-10-24)

The pathogenesis of Acanthamoeba keratitis begins when Acanthamoeba trophozoites bind specifically to mannosylated glycoproteins upregulated on the surfaces of traumatized corneal epithelial cells. When Acanthamoeba castellanii trophozoites are grown in methyl-alpha-D-mannopyranoside, they are induced to secrete a novel 133-kDa protein

Ligand-mediated retargeting of recombinant adenovirus for gene transfer in vivo.

C Thoma et al.

Gene therapy, 7(12), 1039-1045 (2000-06-29)

The development of efficient and safe methods for in vivo gene transfer is central to the success of gene therapy. Recombinant adenoviral vectors, although highly efficient, are limited by the host immune response, potential safety hazards due to obligatory cotransfer

Synthesis, secretion, and subcellular localization of serglycin proteoglycan in human endothelial cells.

B P Schick et al.

Blood, 97(2), 449-458 (2001-01-12)

The serglycin proteoglycan is best known as a hematopoietic cell granule proteoglycan. It has been found that serglycin is synthesized by endothelial cells, is localized to cytoplasmic vesicles, and is constitutively secreted. Serglycin messenger RNA in human umbilical vein endothelial