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A1420

Sigma-Aldrich

Ames′ Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

Ames′ Medium has been formulated to support retinal tissue in relatively short-term culture. It is suitable for maintaining central nervous system tissue in vitro.

Application

Ames′ Medium has been used to maintain rat/mice/macaque monkey eye for the isolation of retina.

Quantity

Formulated to contain 8.8 grams of powder per liter of medium.

Reconstitution

Supplement with 1.9 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

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B Ameline et al.
Gene therapy, 24(11), 735-741 (2017-09-08)
Several preclinical studies have investigated the potential of algal channelrhodopsin and human melanopsin as optogenetic tools for vision restoration. In the present study, we assessed the potentially deleterious effects of long-term expression of these optogenes on the diseased retina in
John M Nagarah et al.
Frontiers in neuroscience, 9, 384-384 (2015-11-06)
Multielectrode arrays (MEAs) allow for acquisition of multisite electrophysiological activity with submillisecond temporal resolution from neural preparations. The signal to noise ratio from such arrays has recently been improved by substrate perforations that allow negative pressure to be applied to
Sabrina Asteriti et al.
Visual neuroscience, 34, E006-E006 (2017-10-03)
Rod-cone gap junctions mediate the so-called "secondary rod pathway", one of three routes that convey rod photoreceptor signals across the retina. Connexin 36 (Cx36) is expressed at these gap junctions, but an unidentified connexin protein also seems to be expressed.
Shijun Weng et al.
Journal of biological rhythms, 24(5), 391-402 (2009-09-17)
Intrinsically photosensitive retinal ganglion cells (ipRGCs) project to the suprachiasmatic nucleus (SCN) and are essential for normal photic entrainment of global circadian rhythms in physiology and behavior. The effect of light on the central clock is dependent on circadian phase
Michael B Manookin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(16), 4136-4150 (2008-04-18)
Cone signals divide into parallel ON and OFF bipolar cell pathways, which respond to objects brighter or darker than the background and release glutamate onto the corresponding type of ganglion cell. It is assumed that ganglion cell excitatory responses are
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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