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Merck
CN

A1731

Aminophenyl fluorescein

Synonym(s):

2-[6-(4′-Amino)phenoxy-3H-xanthen-3-on-9-yl] benzoic acid, APF

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About This Item

Empirical Formula (Hill Notation):
C26H17NO5
Molecular Weight:
423.42
UNSPSC Code:
12352200
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storage condition

protect from light

fluorescence

λex 490 nm; λem 515 nm

storage temp.

2-8°C

Application

Fluorescent detector of highly reactive oxygen species (hROS). Fluorescence intensity increases after reaction with hROS (e.g. hydroxy radical, peroxynitrite and hypochlorite). Since APF does not react with nitric oxide, superoxide, and hydrogen peroxide, it can be used to differentially detect peroxynitrite. APF is resistant to light-induced autoxidation.

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