A2237
Phosphatase, Alkaline shrimp
≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host
Synonym(s):
Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Recombinant:
expressed in proprietary host
Concentration:
≥900 DEA units/mL
Product Name
Phosphatase, Alkaline shrimp, ≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host
recombinant
expressed in proprietary host
form
buffered aqueous glycerol solution
concentration
≥900 DEA units/mL
foreign activity
RNAse, DNAse, none detected
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Biochem/physiol Actions
Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.
Analysis Note
Protein determined by biuret
Application
Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.
General description
Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.
Other Notes
One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.
Physical form
solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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