A278
Monoclonal Anti-Na+/K+ ATPase (β1 Subunit) antibody produced in mouse
clone M17-P5-F11
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About This Item
UNSPSC Code:
12352203
MDL number:
Conjugate:
unconjugated
Clone:
M17-P5-F11, monoclonal
Application:
IHC (f), WB
Citations:
3
conjugate
unconjugated
antibody product type
primary antibodies
clone
M17-P5-F11, monoclonal
form
liquid
mol wt
antigen (kidney) 55 kDa, antigen (liver) 42 kDa
species reactivity
pig, sheep, human, canine
should not react with
rat
technique(s)
immunohistochemistry (frozen sections): 1:100, western blot: 1:1,000-1:10,000
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... ATP1B1(481)
General description
Epitope mapping studies indicate that this clone recognizes the epitope between amino acids 195 and 199 of the sheep β1 subunit of Na+/K+ ATPase. Staining is consistent with the plasma membrane localization of Na+/K+ ATPase.
Mouse monoclonal anti-Na+/K+ ATPase (β1 subunit) reacts with the β1 subunit of Na+/K+ ATPase in humans, sheep, canines and pigs, but not in rats. The product shows no reactivity with rat β2 subunit. In immunoblotting, the antibody detects the β1 subunit of Na+/K+ ATPase as a 42kDa band in liver samples, and as a 52kDa band in kidney samples.
Na+/K+ transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.
Immunogen
lamb kidney β subunit of Na+/K+ ATPase.
Application
Mouse Monoclonal Anti-Na+/K+ ATPase (β1 subunit) can be used in immunohistochemical (1:100) and western blot (1:1,000 - 1:10,000) applications
Physical form
Solution in phosphate buffered saline with 1mg/ml BSA and 0.05% sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
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Ya Su et al.
Scientific reports, 7, 39701-39701 (2017-01-04)
Anion exchanger 1 (AE1) mediates Cl-/HCO3- exchange in erythrocytes and kidney intercalated cells where it functions to maintain normal bodily acid-base homeostasis. AE1's C-terminal tail (AE1C) contains multiple potential membrane targeting/retention determinants, including a predicted PDZ binding motif, which are
Chia-Chi Liu et al.
Frontiers in oncology, 12, 859216-859216 (2022-04-05)
The seven mammalian FXYD proteins associate closely with α/β heterodimers of Na+/K+-ATPase. Most of them protect the β1 subunit against glutathionylation, an oxidative modification that destabilizes the heterodimer and inhibits Na+/K+-ATPase activity. A specific cysteine (Cys) residue of FXYD proteins
Ya Su et al.
Journal of the American Society of Nephrology : JASN, 26(2), 400-409 (2014-07-12)
Anion exchanger-1 (AE1) mediates chloride-bicarbonate exchange across the plasma membranes of erythrocytes and, via a slightly shorter transcript, kidney epithelial cells. On an omnivorous human diet, kidney AE1 is mainly active basolaterally in α-intercalated cells of the collecting duct, where
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